Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 72,229 C→T *233* (TAG→TAA thiQ ← thiamine/thiamine pyrophosphate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091372,2290CT100.0% 97.9 / NA 31*233* (TAG→TAAthiQthiamine/thiamine pyrophosphate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/16);  total (15/16)

AAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACG  >  NC_000913/72098‑72361
                                                                                                                                   |                                                                                                                                    
aaaGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  1:314008/139‑1 (MQ=255)
aaaGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:657476/139‑1 (MQ=255)
           ggTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAAt                                                                                                                    >  1:287564/1‑139 (MQ=255)
             tttAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAg                                                                                                                  <  2:589847/139‑1 (MQ=255)
              ttAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGt                                                                                                                 >  1:542647/1‑139 (MQ=255)
                                       ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTca                                                                                        >  2:586269/1‑139 (MQ=255)
                                        cTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTcaa                                                                                       <  2:542647/139‑1 (MQ=255)
                                         tGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTcaac                                                                                      <  1:385417/139‑1 (MQ=255)
                                               gACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  2:850841/139‑1 (MQ=255)
                                                            tCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCt                                                                   >  2:322353/1‑139 (MQ=255)
                                                                    ggCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               >  1:287003/1‑71 (MQ=255)
                                                                    ggCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:287003/71‑1 (MQ=255)
                                                                                     aCCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCt                                                                                          >  1:137679/1‑91 (MQ=255)
                                                                                     aCCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCt                                                                                          <  2:137679/91‑1 (MQ=255)
                                                                                     aCCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCACTCAACAACTCAt                                                                               <  1:566436/102‑1 (MQ=255)
                                                                                     aCCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCACTCAACAACTCAt                                                                               >  2:566436/1‑102 (MQ=255)
                                                                                       ccGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGacta                                        >  2:128823/1‑139 (MQ=255)
                                                                                                ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               <  2:617954/43‑1 (MQ=255)
                                                                                                ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                               >  1:617954/1‑43 (MQ=255)
                                                                                                ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgc                               >  1:422138/1‑139 (MQ=255)
                                                                                                 gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                >  2:844881/1‑89 (MQ=255)
                                                                                                 gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                                <  1:844881/89‑1 (MQ=255)
                                                                                                 gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCAt                                                                               <  2:188633/90‑1 (MQ=255)
                                                                                                 gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCAt                                                                               >  1:188633/1‑90 (MQ=255)
                                                                                                          ttCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGCTGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGAt                     >  1:32316/1‑139 (MQ=255)
                                                                                                                   cATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGcgccgc            <  1:586269/139‑1 (MQ=255)
                                                                                                                     tCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCAt          >  1:754957/1‑139 (MQ=255)
                                                                                                                             tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCAc                                                                                                       <  2:53248/38‑1 (MQ=255)
                                                                                                                             tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCAc                                                                                                       >  1:53248/1‑38 (MQ=255)
                                                                                                                             tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGTGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACg  <  1:322353/139‑1 (MQ=255)
                                                                                                                             tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACg  <  2:422138/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                    
AAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACG  >  NC_000913/72098‑72361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: