Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 88,325 G→A E100K (GAA→AAA)  cra → transcriptional repressor‑activator for carbon metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091388,3250GA100.0% 160.1 / NA 50E100K (GAA→AAA) cratranscriptional repressor‑activator for carbon metabolism
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/25);  total (25/25)

CTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTTCCCGATT  >  NC_000913/88193‑88459
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cTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATc                                                                                                                                  >  1:145162/1‑139 (MQ=255)
     tGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCa                                                                                                                             <  2:145162/139‑1 (MQ=255)
               acacGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaaa                                                                                                                   >  2:183272/1‑139 (MQ=255)
                   gTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCg                                                                                                               <  2:319217/139‑1 (MQ=255)
                         ttGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCAt                                                                                                         >  2:325002/1‑139 (MQ=255)
                                 gtgATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCAcc                                                                                                 >  2:858212/1‑139 (MQ=255)
                                      ccccGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                    >  2:700623/1‑115 (MQ=255)
                                      ccccGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                    <  1:700623/115‑1 (MQ=255)
                                      ccccGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa                                                                                            <  1:268078/139‑1 (MQ=255)
                                      ccccGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa                                                                                            <  1:858212/139‑1 (MQ=255)
                                        ccGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACa                                                                                          <  1:183272/139‑1 (MQ=255)
                                                          gCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCat                                                                        >  1:349802/1‑139 (MQ=255)
                                                          gCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCat                                                                        >  1:665373/1‑139 (MQ=255)
                                                               aCCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGc                                                                                                           >  2:43465/1‑99 (MQ=255)
                                                               aCCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGc                                                                                                           <  1:43465/99‑1 (MQ=255)
                                                                cccGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGt                                                                  >  2:244225/1‑139 (MQ=255)
                                                                    cATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGAt                                                                             >  1:66117/1‑124 (MQ=255)
                                                                    cATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGAt                                                                             <  2:66117/124‑1 (MQ=255)
                                                                     aTCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGATAACGAAATGCGGTGCATTGAg                                                                                                     <  1:630509/99‑1 (MQ=255)
                                                                     aTCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCa                                                                                                          >  1:474798/1‑94 (MQ=255)
                                                                     aTCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCa                                                                                                          <  2:474798/94‑1 (MQ=255)
                                                                     aTCGCTAACTATCTTGAACGCCAGACGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGATAACGAAATGCGGTGCATTGAg                                                                                                     >  2:630509/1‑99 (MQ=255)
                                                                      tCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCg                                                                                                               >  1:825651/1‑88 (MQ=255)
                                                                      tCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCg                                                                                                               <  2:825651/88‑1 (MQ=255)
                                                                           aaCTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGAcgtcgt                                                       <  2:663912/139‑1 (MQ=255)
                                                                            aCTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGtt                                                      <  2:828174/139‑1 (MQ=255)
                                                                            aCTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGtt                                                      <  2:724283/139‑1 (MQ=255)
                                                                                 cTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGcctc                                                 >  2:933271/1‑139 (MQ=255)
                                                                                 cTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGACAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGcctc                                                 >  2:506214/1‑139 (MQ=255)
                                                                                          cAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACAAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGa                                                                              >  2:426174/1‑101 (MQ=255)
                                                                                          cAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACAAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGa                                                                              <  1:426174/101‑1 (MQ=255)
                                                                                               gcgGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                    >  2:914014/1‑58 (MQ=255)
                                                                                               gcgGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGaa                                                                                                                    <  1:914014/58‑1 (MQ=255)
                                                                                                  gCAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTAt                                <  2:349802/139‑1 (MQ=255)
                                                                                                   cAACGGGGTTATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATc                               <  1:325002/139‑1 (MQ=255)
                                                                                                           ttATCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTgg                       >  2:358968/1‑139 (MQ=255)
                                                                                                             aTCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGa                                                                              >  2:724316/1‑82 (MQ=255)
                                                                                                             aTCAACTGCTGATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGa                                                                              <  1:724316/82‑1 (MQ=255)
                                                                                                                    gctgATTGCCTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGAc              <  1:358968/139‑1 (MQ=255)
                                                                                                                     ctgATTGACTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTATACATCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTACTGAGCATCCTTTTTATCGGCGCTGGGCTAACGAcc             <  2:665373/139‑1 (MQ=255)
                                                                                                                            ccTGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTTccc      >  1:465840/1‑139 (MQ=255)
                                                                                                                              tGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                      >  2:576915/1‑41 (MQ=255)
                                                                                                                              tGCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                      <  1:576915/41‑1 (MQ=255)
                                                                                                                               gCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                      <  1:580589/40‑1 (MQ=255)
                                                                                                                               gCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                                                                                                      >  2:580589/1‑40 (MQ=255)
                                                                                                                               gCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCAcc                                                                                                 >  1:48589/1‑45 (MQ=255)
                                                                                                                               gCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCAcc                                                                                                 <  2:48589/45‑1 (MQ=255)
                                                                                                                               gCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa                                                                                            >  1:331869/1‑50 (MQ=255)
                                                                                                                               gCTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa                                                                                            <  2:331869/50‑1 (MQ=255)
                                                                                                                                cTCAAAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTTCCCGAtt  >  1:684855/1‑139 (MQ=255)
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CTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGACCCGTTCCCGATT  >  NC_000913/88193‑88459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑