Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,128,134 C→T T99M (ACG→ATG)  murJ → putative lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,128,1340CT100.0% 122.4 / NA 39T99M (ACG→ATG) murJputative lipid II flippase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/21);  total (18/21)

TTTGCCGAAGGGGCATTTTCCCAGGCATTTGTACCGATTCTGGCGGAATATAAAAGTAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGACGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCC  >  NC_000913/1128001‑1128264
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tttGCCGAAGGGGCATTTTCCCAGGCATTTGTACCGATTCTGGCGGAATATAAAAGTAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCg                                                                                                                               <  2:339497/139‑1 (MQ=255)
       aaGGGGCATTTTCCCAGGCATTTGTACCGATTCTGGCGGAATATAAAAGTAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCAt                                                                                                                        >  2:240177/1‑139 (MQ=255)
       aaGGGGCATTTTCCCAGGCATTTGTACCGATTCTGGCGGAATATAAAAGTAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCAt                                                                                                                        <  1:348497/139‑1 (MQ=255)
                  tCCCAGGCATTTGTACCGATTCTGGCGGAATATAAAAGTAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCAc                                                                                                             <  1:413583/139‑1 (MQ=255)
                   cccAGGCATTTGTACCGATTCTGGCGGAATATAAAAGTAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCa                                                                                                              >  1:154997/1‑137 (MQ=255)
                   cccAGGCATTTGTACCGATTCTGGCGGAATATAAAAGTAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCa                                                                                                              <  2:154997/137‑1 (MQ=255)
                          aTTTGTACCGATTCTGGCGGAATATAAAAGTAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTg                                                                                                     <  2:548773/139‑1 (MQ=255)
                            ttGTACCGATTCTGGCGGAATATAAAAGTAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCAc                                                                                                             <  1:415934/129‑1 (MQ=255)
                            ttGTACCGATTCTGGCGGAATATAAAAGTAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCAc                                                                                                             >  2:415934/1‑129 (MQ=255)
                                                        tAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGt                                                                                                                                 <  2:786103/81‑1 (MQ=255)
                                                        tAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGt                                                                                                                                 >  1:786103/1‑81 (MQ=255)
                                                        tAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGc                                                                                >  2:564145/1‑130 (MQ=255)
                                                        tAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGc                                                                                <  1:564145/130‑1 (MQ=255)
                                                          aGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGacac                                                                     >  2:837381/1‑139 (MQ=255)
                                                                     gACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAAt                                                          <  1:240177/139‑1 (MQ=255)
                                                                         ccACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGc                                                      >  2:134660/1‑139 (MQ=255)
                                                                                  tCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGGTGGCGGTTGTGATGGTCAGTAGCATGCTCGCCGCAACGAAGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACAGACCTGACAAATTTGCCCTGGccag                                             >  2:305832/1‑139 (MQ=255)
                                                                                    tttGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGcc                                                     <  1:297261/129‑1 (MQ=255)
                                                                                    tttGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGcc                                                     >  2:297261/1‑129 (MQ=255)
                                                                                       gTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTggg                                                                                                       <  1:423343/76‑1 (MQ=255)
                                                                                       gTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTggg                                                                                                       >  2:423343/1‑76 (MQ=255)
                                                                                                     tGGCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTAcc                          <  1:134660/139‑1 (MQ=255)
                                                                                                      ggCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGAccag                                             <  1:218430/119‑1 (MQ=255)
                                                                                                      ggCCTGCTGACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGAccag                                             >  2:218430/1‑119 (MQ=255)
                                                                                                            ctgACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGt                                                                                                          >  2:556197/1‑52 (MQ=255)
                                                                                                            ctgACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGt                                                                                                          <  1:556197/52‑1 (MQ=255)
                                                                                                            ctgACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGcc                                                     <  2:15585/105‑1 (MQ=255)
                                                                                                            ctgACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGcc                                                     >  1:15585/1‑105 (MQ=255)
                                                                                                            ctgACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCt                   <  1:837381/139‑1 (MQ=255)
                                                                                                            ctgACACTTGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCt                   <  2:795889/139‑1 (MQ=255)
                                                                                                                    tGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTAcc                          >  2:471650/1‑124 (MQ=255)
                                                                                                                    tGCGCTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTAcc                          <  1:471650/124‑1 (MQ=255)
                                                                                                                      cgcTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAg                                                                   >  2:511298/1‑81 (MQ=255)
                                                                                                                      cgcTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAg                                                                   <  1:511298/81‑1 (MQ=255)
                                                                                                                      cgcTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGcc                                                     >  1:517735/1‑95 (MQ=255)
                                                                                                                      cgcTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGcc                                                     <  2:517735/95‑1 (MQ=255)
                                                                                                                        cTGGCGGTTGTGATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGa       >  2:257533/1‑139 (MQ=255)
                                                                                                                                 gtgATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTcc  >  2:31702/1‑135 (MQ=255)
                                                                                                                                 gtgATGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTcc  <  1:31702/135‑1 (MQ=255)
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TTTGCCGAAGGGGCATTTTCCCAGGCATTTGTACCGATTCTGGCGGAATATAAAAGTAAGCAGGGTGAAGACGCCACGCGGGTCTTTGTCTCTTATGTTTCTGGCCTGCTGACACTTGCGCTGGCGGTTGTGACGGTCGCTGGCATGCTCGCCGCACCGTGGGTGATCATGGTGACCGCGCCAGGCTTCGCTGACACAGCTGACAAATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCC  >  NC_000913/1128001‑1128264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑