Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,208,517 C→T P129L (CCT→CTT) 
*201* (TAG→TAA
tfaP →
tfaE ←
e14 prophage, uncharacterized protein
e14 prophage, putative tail fiber assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,208,5170CT100.0% 151.7 / NA 49P129L (CCT→CTT) 
*201* (TAG→TAA
tfaP
tfaE
e14 prophage, uncharacterized protein
e14 prophage, putative tail fiber assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/24);  total (25/24)

AGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGG  >  NC_000913/1208391‑1208644
                                                                                                                              |                                                                                                                               
aGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                       <  1:702827/137‑1 (MQ=255)
aGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                       >  2:702827/1‑137 (MQ=255)
  tGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAg                                                                                                                   >  1:774770/1‑139 (MQ=255)
            aGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGa                                                                                                        >  2:784381/1‑139 (MQ=255)
               cTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGa                                                                                                     >  2:629735/1‑139 (MQ=255)
                  cATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATg                                                                                                      <  2:629085/135‑1 (MQ=255)
                  cATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTAACGCCTCCGGCAGTTAGGCCAGATg                                                                                                      >  1:629085/1‑135 (MQ=255)
                       ggAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGACAGTTAGGCCAGATGACATCCGgc                                                                                             >  1:833851/1‑139 (MQ=255)
                        gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                            <  1:858264/139‑1 (MQ=255)
                          aGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGcgcg                                                                                          <  2:906396/139‑1 (MQ=255)
                                aaaGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTg                                                                                    <  1:197528/139‑1 (MQ=255)
                                      gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGAt                                                                                                       <  1:114319/114‑1 (MQ=255)
                                      gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGAt                                                                                                       >  2:114319/1‑114 (MQ=255)
                                       aTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                       >  2:476682/1‑98 (MQ=255)
                                       aTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                       <  1:476682/98‑1 (MQ=255)
                                                     tataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTCCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACcgcg                                                               >  1:55436/1‑139 (MQ=255)
                                                      ataATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                              <  1:246159/139‑1 (MQ=255)
                                                           ttGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         <  1:860180/139‑1 (MQ=255)
                                                             gtgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGt                                                                                  >  1:545236/1‑112 (MQ=255)
                                                             gtgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGt                                                                                  <  2:545236/112‑1 (MQ=255)
                                                               ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGcgcg                                                                                          <  1:340941/102‑1 (MQ=255)
                                                               ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGcgcg                                                                                          >  2:340941/1‑102 (MQ=255)
                                                                    gATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGc                                                >  1:247248/1‑139 (MQ=255)
                                                                                gCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGt                                    <  2:774770/139‑1 (MQ=255)
                                                                                  aCTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGACAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCt                                  <  2:833851/139‑1 (MQ=255)
                                                                                   cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                            <  1:472986/80‑1 (MQ=17)
                                                                                   cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                            >  2:472986/1‑80 (MQ=17)
                                                                                   cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCa                                                                    >  1:719361/1‑104 (MQ=255)
                                                                                   cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCa                                                                    <  2:719361/104‑1 (MQ=255)
                                                                                            gTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                       >  2:685961/1‑45 (MQ=255)
                                                                                            gTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                       <  1:685961/45‑1 (MQ=255)
                                                                                               gATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTctgcc                     <  2:65512/139‑1 (MQ=255)
                                                                                                 tACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGtt                                        >  2:432551/1‑118 (MQ=255)
                                                                                                 tACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGtt                                        <  1:432551/118‑1 (MQ=255)
                                                                                                 tACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTctgcctg                   >  1:290201/1‑139 (MQ=255)
                                                                                                    aTCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                >  2:240974/1‑139 (MQ=255)
                                                                                                      ccAGTGCGCCAGATATTGAATGGCTTACGTCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTgg                                >  2:352979/1‑121 (MQ=255)
                                                                                                      ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         <  2:172403/96‑1 (MQ=255)
                                                                                                      ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         >  1:172403/1‑96 (MQ=255)
                                                                                                      ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTgg                                <  1:352979/121‑1 (MQ=255)
                                                                                                      ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCa              >  2:263657/1‑139 (MQ=255)
                                                                                                      ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCa              >  1:373114/1‑139 (MQ=255)
                                                                                                          tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                >  2:162583/1‑133 (MQ=255)
                                                                                                          tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                <  1:162583/133‑1 (MQ=255)
                                                                                                            cgcCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                              <  2:491687/85‑1 (MQ=255)
                                                                                                            cgcCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                              >  1:491687/1‑85 (MQ=255)
                                                                                                                  atatTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGTGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCgg  <  2:464466/139‑1 (MQ=255)
                                                                                                                         aTGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTg                                                                                       <  2:303608/47‑1 (MQ=11)
                                                                                                                         aTGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTg                                                                                       >  1:303608/1‑47 (MQ=14)
                                                                                                                              |                                                                                                                               
AGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGG  >  NC_000913/1208391‑1208644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: