Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,327,261 A→G E137G (GAA→GGA)  rluB → 23S rRNA pseudouridine(2605) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,327,2610AG100.0% 171.3 / NA 48E137G (GAA→GGA) rluB23S rRNA pseudouridine(2605) synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/24);  total (24/24)

TTTGACCGTCTGCCAAAACTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGAACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAC  >  NC_000913/1327125‑1327394
                                                                                                                                        |                                                                                                                                     
tttGACCGTCTGCCAAAACTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGAc                                                                                                                                     >  1:26458/1‑139 (MQ=255)
     ccGTCTGCCAAAACTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGaa                                                                                                                                <  1:323966/139‑1 (MQ=255)
                aaCTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg                                                                                                                     <  2:414100/139‑1 (MQ=255)
                aaCTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg                                                                                                                     <  1:885287/139‑1 (MQ=255)
                          cgcACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGt                                                                                                           <  1:797404/139‑1 (MQ=255)
                                cTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGtt                                                                                                     <  2:488060/139‑1 (MQ=255)
                                    aTTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTgacg                                                                                                 >  1:701208/1‑139 (MQ=255)
                                                                   gtgGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGt                                                                  >  2:509035/1‑139 (MQ=255)
                                                                    tgGTCTGCTGCTGTTCACCACCGACGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTg                                                                 <  2:264580/139‑1 (MQ=255)
                                                                    tgGTCTGCCGCTGTTCACAAACGATTGTGAACTGGCAAACCGTTTAATGCTCCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTg                                                                 <  2:701208/139‑1 (MQ=255)
                                                                         tgctgctgTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgc                                                                                                                      <  2:705133/81‑1 (MQ=255)
                                                                         tgctgctgTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgc                                                                                                                      >  1:705133/1‑81 (MQ=255)
                                                                           ctgctgTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTga                                                                                                   >  2:19971/1‑98 (MQ=255)
                                                                           ctgctgTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTga                                                                                                   <  1:19971/98‑1 (MQ=255)
                                                                                 ttCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATg                                                    >  1:316765/1‑139 (MQ=255)
                                                                                     ccaccGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGaa                                                                                                                                <  1:672845/59‑1 (MQ=255)
                                                                                     ccaccGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGaa                                                                                                                                >  2:672845/1‑59 (MQ=255)
                                                                                     ccaccGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTcc                                                >  1:861339/1‑139 (MQ=255)
                                                                                           aTGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTg                                                                 >  1:636271/1‑116 (MQ=255)
                                                                                           aTGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTg                                                                 <  2:636271/116‑1 (MQ=255)
                                                                                           aTGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGc                                          >  2:56408/1‑139 (MQ=255)
                                                                                           aTGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGc                                          >  1:711488/1‑139 (MQ=255)
                                                                                                      gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg                                                                                                                     <  1:348180/53‑1 (MQ=255)
                                                                                                      gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg                                                                                                                     >  2:348180/1‑53 (MQ=255)
                                                                                                      gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGt                                                                                                           >  2:931116/1‑63 (MQ=255)
                                                                                                      gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGt                                                                                                           <  1:931116/63‑1 (MQ=255)
                                                                                                      gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGAt                                                                                <  2:10102/90‑1 (MQ=255)
                                                                                                      gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGAt                                                                                >  1:10102/1‑90 (MQ=255)
                                                                                                      gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTc                                                                         >  1:643628/1‑97 (MQ=255)
                                                                                                      gCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTc                                                                         <  2:643628/97‑1 (MQ=255)
                                                                                                              tttAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTc                       >  1:141500/1‑139 (MQ=255)
                                                                                                              tttAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTc                       >  1:441527/1‑139 (MQ=255)
                                                                                                                tAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg                                                                                                                     >  1:598127/1‑43 (MQ=255)
                                                                                                                tAATGCACCCAAGCCGTGAAGTTGGACGTGAATATGCcgtgcg                                                                                                                     <  2:598127/43‑1 (MQ=255)
                                                                                                                   tGCACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAgcgg                  >  1:5042/1‑139 (MQ=255)
                                                                                                                     cACCCAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAgcggcg                <  1:509035/139‑1 (MQ=255)
                                                                                                                        ccAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAgcggcggcg             >  1:312402/1‑139 (MQ=255)
                                                                                                                        ccAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAgcggcggcg             >  2:202420/1‑139 (MQ=255)
                                                                                                                         cAAGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGa            <  2:26458/139‑1 (MQ=255)
                                                                                                                           aGCCGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAg          >  1:316161/1‑139 (MQ=255)
                                                                                                                             ccGTGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAggg        >  1:229024/1‑139 (MQ=255)
                                                                                                                                tGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCg                                                                   <  1:530986/77‑1 (MQ=255)
                                                                                                                                tGAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCg                                                                   >  2:530986/1‑77 (MQ=255)
                                                                                                                                 gAAGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCa    <  1:169410/139‑1 (MQ=255)
                                                                                                                                   aGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAc  <  2:229024/139‑1 (MQ=255)
                                                                                                                                   aGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAc  <  1:202420/139‑1 (MQ=255)
                                                                                                                                   aGTTGGACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAc  <  2:141500/139‑1 (MQ=255)
                                                                                                                                   aGTTGGACGTGAATATGCCGTGCGTGTATTTGGGCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAc  <  2:441527/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TTTGACCGTCTGCCAAAACTGCGTGGCGCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACCACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGAACGTGAATATGCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTGCAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATCAAC  >  NC_000913/1327125‑1327394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑