Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,382,141 A→G R65R (AGA→AGG)  ompG → outer membrane porin G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,382,1410AG100.0% 161.0 / NA 45R65R (AGA→AGG) ompGouter membrane porin G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/23);  total (22/23)

AAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCATTATCAGTTCCTCGAAAACGATGATTTCAG  >  NC_000913/1382014‑1382260
                                                                                                                               |                                                                                                                       
aaaggaaCGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc                                                                                                              <  1:402939/139‑1 (MQ=255)
 aaggaaCGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGcc                                                                                                             <  1:193820/139‑1 (MQ=255)
         aCTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCa                                                                                                     >  2:375320/1‑139 (MQ=255)
                 tttAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTGTCAGGAAGGGc                                                                                             <  1:826565/139‑1 (MQ=255)
                 tttAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGc                                                                                             <  2:332001/139‑1 (MQ=255)
                 tttAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGc                                                                                             <  2:456899/139‑1 (MQ=255)
                        tCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGa                                                                                      >  2:889506/1‑139 (MQ=255)
                           gcgcGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc                                                                                                              >  1:18760/1‑112 (MQ=255)
                           gcgcGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc                                                                                                              <  2:18760/112‑1 (MQ=255)
                                           aGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCTGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAaacgt                                                                   >  2:56673/1‑139 (MQ=255)
                                                  gTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc                                                                                                              >  2:69510/1‑89 (MQ=255)
                                                  gTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc                                                                                                              <  1:69510/89‑1 (MQ=255)
                                                   tCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGct                                                                                                                   <  2:419331/83‑1 (MQ=255)
                                                   tCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGct                                                                                                                   >  1:419331/1‑83 (MQ=255)
                                                      aGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGct                                                                                                                   <  1:26784/80‑1 (MQ=255)
                                                      aGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGct                                                                                                                   >  2:26784/1‑80 (MQ=255)
                                                                   aGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCt                                                                                                            >  1:241595/1‑74 (MQ=255)
                                                                   aGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCt                                                                                                            <  2:241595/74‑1 (MQ=255)
                                                                   aGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAg                                           <  1:889506/139‑1 (MQ=255)
                                                                    gATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGc                                          <  2:530353/139‑1 (MQ=255)
                                                                         ggatggGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAg                                     >  1:413805/1‑139 (MQ=255)
                                                                               gCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCg                                                                                           >  2:154641/1‑79 (MQ=255)
                                                                               gCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCg                                                                                           <  1:154641/79‑1 (MQ=255)
                                                                               gCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCAt                               >  1:214806/1‑139 (MQ=255)
                                                                               gCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCAt                               >  2:528881/1‑139 (MQ=255)
                                                                               gCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCAt                               >  2:30019/1‑139 (MQ=255)
                                                                                cTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGa                                      >  2:252443/1‑131 (MQ=255)
                                                                                cTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGa                                      <  1:252443/131‑1 (MQ=255)
                                                                                cTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCAtt                              >  2:838718/1‑139 (MQ=255)
                                                                                   gCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAg                                                                                >  1:56337/1‑86 (MQ=255)
                                                                                   gCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAg                                                                                <  2:56337/86‑1 (MQ=255)
                                                                                    cGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc                                                                                                              >  1:757335/1‑55 (MQ=255)
                                                                                    cGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc                                                                                                              <  2:757335/55‑1 (MQ=255)
                                                                                         ccTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGcc                                                <  1:137111/112‑1 (MQ=255)
                                                                                         ccTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGcc                                                >  2:137111/1‑112 (MQ=255)
                                                                                                  tATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc                                                                                                              <  1:549452/41‑1 (MQ=255)
                                                                                                  tATTTTAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGc                                                                                                              >  2:549452/1‑41 (MQ=255)
                                                                                                    ttttAATGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCATTATCAGTTCCTCGAAAACgat          <  2:413805/139‑1 (MQ=255)
                                                                                                         aTGCCGCCAACGGGCGGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGAGGGTAAAAGTGGAGCGGGGTTTGTTCGCCCGGAGCTGGAGGTGCAGTATCACTTCCTCGAAAACGATGAttt     <  2:851750/139‑1 (MQ=255)
                                                                                                         aTGCCGCCAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCATTATCAGTTCCTCGAAAACGATGAttt     <  1:375320/139‑1 (MQ=255)
                                                                                                            ccgccAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCATTATCAGTTCCTCGAAAACGATGATTTCAg  >  1:535902/1‑139 (MQ=255)
                                                                                                              gccAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTgg                                       >  1:243418/1‑100 (MQ=255)
                                                                                                              gccAACGGGCCGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTgg                                       <  2:243418/100‑1 (MQ=255)
                                                                                                                        cGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGcgcg                                                                            <  1:183404/53‑1 (MQ=255)
                                                                                                                        cGTGGAGGATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGcgcg                                                                            >  2:183404/1‑53 (MQ=255)
                                                                                                                               |                                                                                                                       
AAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGCCTATTATCAGGAAGGGCCGGTAGATTATAGCGCGGGTAAACGTGGAACGTGGTTTGATCGCCCGGAGCTGGAGGTGCATTATCAGTTCCTCGAAAACGATGATTTCAG  >  NC_000913/1382014‑1382260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑