Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,389,895	G→A	*322* (TAG→TAA)  P235L (CCT→CTT)	ycjG → mpaA ←	L‑Ala‑D/L‑Glu epimerase murein peptide amidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,389,895	0	G	A	100.0%	62.3 / NA	21	*322* (TAG→TAA)  P235L (CCT→CTT)	ycjG mpaA	L‑Ala‑D/L‑Glu epimerase murein peptide amidase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/10);  total (11/10)

TAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAGGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGC  >  NC_000913/1389778‑1390021                                                                                                                      |                                                                                                                               tAGCGCCGCTTTACCGCTGGAGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCTGCAAGttgt                                                                                                           >  2:547401/1‑137 (MQ=255)   gcgcCGCTTTACCGCTGGTGCAGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGcc                                                                                                         <  1:547401/139‑1 (MQ=255)      ccgcTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATc                                                                                                      >  2:343519/1‑139 (MQ=255)                   ggTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATAtt                                                                                         >  1:305935/1‑139 (MQ=255)                                       ccGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGc                                                                                                          >  1:328835/1‑102 (MQ=255)                                       ccGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGc                                                                                                          <  2:328835/102‑1 (MQ=255)                                                   gaccgaccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGa                                                         <  2:305935/139‑1 (MQ=255)                                                   gaccgaccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGa                                                         <  1:338360/139‑1 (MQ=255)                                                     ccgaccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCgca                                                                                                                    >  1:433909/1‑78 (MQ=255)                                                     ccgaccTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCgca                                                                                                                    <  2:433909/78‑1 (MQ=255)                                                          ctggctggCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTg                                                  <  2:500491/139‑1 (MQ=255)                                                                        aTGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGatgcaatgc                                    <  2:726242/139‑1 (MQ=255)                                                                                   gcgcTTCAGTTCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATc                         >  2:247926/1‑139 (MQ=255)                                                                                             tCACGACGGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCaa               >  2:789403/1‑139 (MQ=255)                                                                                                 gacgGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGcc                                                                                                         <  1:781041/45‑1 (MQ=255)                                                                                                 gacgGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGcc                                                                                                         >  2:781041/1‑45 (MQ=255)                                                                                                 gacgGGCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGc           >  1:73749/1‑139 (MQ=255)                                                                                                     ggCGAATTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGaa       <  1:343519/139‑1 (MQ=255)                                                                                                          aTTGCATCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGc  >  2:798509/1‑139 (MQ=255)                                                                                                                tCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGCGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCCGATc                         >  1:428291/1‑110 (MQ=255)                                                                                                                tCTTTAAGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATc                         <  2:428291/110‑1 (MQ=255)                                                                                                                      |                                                                                                                               TAGCGCCGCTTTACCGCTGGTGCCGCAGGTCAGTTTCGCCGATCTTGACGGACCGACCTGGCTGGCGGTAGATGTGGAACCGGCGCTTCAGTTCACGACGGGCGAATTGCATCTTTAGGATGCCAGCGCAGCAAGTTTGCCATCGCAAAGAGATATTTTTCGCTGGCTTCGTCGGAGGAGATGGGCGGAAACTCTGCGGTGATGCAATGCAAGTTCAGATCTGCACACCAACTGCCGAAAGAGC  >  NC_000913/1389778‑1390021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑