Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 152,036 C→T V66I (GTA→ATA)  yadL ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913152,0360CT100.0% 126.0 / NA 42V66I (GTA→ATA) yadLputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/25);  total (17/25)

TCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGC  >  NC_000913/151911‑152164
                                                                                                                             |                                                                                                                                
tCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATca                                                                                                                     >  2:824524/1‑139 (MQ=255)
 cccTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaa                                                                                                                    <  1:145244/139‑1 (MQ=255)
    tCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaacaa                                                                                                                 >  1:429038/1‑139 (MQ=255)
         tgtgGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                            <  2:618580/139‑1 (MQ=255)
            ggCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATg                                                                                                         >  1:89475/1‑139 (MQ=255)
             gCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                            >  1:664664/1‑135 (MQ=255)
             gCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                            <  2:664664/135‑1 (MQ=255)
              cTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTc                                                                                                       <  1:569996/139‑1 (MQ=255)
                     tgTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGTACAGTCTTTGATTTTGAGTTTGAATGTTTTTACTTTCGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCa                                                                                                <  2:160892/138‑1 (MQ=255)
                     ggTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCa                                                                                                <  2:429038/139‑1 (MQ=255)
                     ggTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCa                                                                                                <  1:824524/139‑1 (MQ=255)
                     ggTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCa                                                                                                <  2:575749/139‑1 (MQ=255)
                                           ttttttgggATACCCCCACAGTCTTTTAATTTTAGTTTGAATGTTTTTTCTTTGGTCTTGGCATTGAATTCCGAAATATAAATAACCCCAAAAACAACAACAGGAATGTCCTTGTCAGAGTCAATTACAAAGGTAGccg                                                                          <  2:789865/133‑3 (MQ=255)
                                           ttATTTGGGATTCCCGCACATTCTTTGAATTTTAATTTGACTGTTTTTACTTTGGTCTTGGCATTGAATTCACAAATATATATATCACCAAAATCAACAGCAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCgg                                                                          <  2:713174/139‑1 (MQ=255)
                                                   gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                                  >  1:288782/1‑139 (MQ=255)
                                                     tACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAcc                                                                <  2:125551/139‑1 (MQ=255)
                                                             caGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                        <  2:284222/139‑1 (MQ=255)
                                                                tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAg                                                                                                              <  2:95711/82‑1 (MQ=255)
                                                                tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAg                                                                                                              >  1:95711/1‑82 (MQ=255)
                                                                tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACag                                                     >  2:355694/1‑139 (MQ=255)
                                                                tCTTTGAATTTGAGTTTGAATGCTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACag                                                     >  1:397702/1‑139 (MQ=255)
                                                                gcTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATCTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGAACAGAGTCATTTACTAATGTAGCGGTACAAGTAACCATTTCTACag                                                     >  2:671115/2‑139 (MQ=255)
                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTAc                                                                                     <  2:709259/97‑1 (MQ=255)
                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTAc                                                                                     >  1:709259/1‑97 (MQ=255)
                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc                                                       <  2:690390/127‑1 (MQ=255)
                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc                                                       >  1:690390/1‑127 (MQ=255)
                                                                          tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAAc                                           >  2:243439/1‑139 (MQ=255)
                                                                             gTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                        <  2:288782/139‑1 (MQ=255)
                                                                             gTTTAAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAACGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                        <  1:322898/139‑1 (MQ=255)
                                                                                tGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAg                                     <  1:258133/139‑1 (MQ=255)
                                                                                               tGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTc                                                                                           >  1:753164/1‑70 (MQ=255)
                                                                                               tGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTc                                                                                           <  2:753164/70‑1 (MQ=255)
                                                                                               tGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                        <  1:872383/121‑1 (MQ=255)
                                                                                               tGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                        >  2:872383/1‑121 (MQ=255)
                                                                                                ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACagtag                                                  >  2:500364/1‑110 (MQ=255)
                                                                                                ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACagtag                                                  <  1:500364/110‑1 (MQ=255)
                                                                                                      ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                                  <  1:81852/88‑1 (MQ=255)
                                                                                                      ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                                  >  2:81852/1‑88 (MQ=255)
                                                                                                      ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCa               <  1:370154/139‑1 (MQ=255)
                                                                                                         aTTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                        >  1:912381/1‑95 (MQ=255)
                                                                                                         aTTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                        <  2:912381/95‑1 (MQ=255)
                                                                                                                   gAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGc  <  2:89475/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                                
TCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGC  >  NC_000913/151911‑152164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: