Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,606,561 A→G K154K (AAA→AAG lsrF → putative autoinducer‑2 (AI‑2) aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,606,5610AG100.0% 96.2 / NA 27K154K (AAA→AAGlsrFputative autoinducer‑2 (AI‑2) aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)

CGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCG  >  NC_000913/1606431‑1606679
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cGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTGTCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAAtattat                                                                                                                >  1:648173/1‑139 (MQ=255)
               cGGAATTAAGTAATGAAGACGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGTGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGc                                                                                                 <  1:626559/139‑1 (MQ=255)
                    ttAAGTAATGAAGCCGTGGCGTTGTCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGaa                                                                                            <  2:648173/139‑1 (MQ=255)
                        gTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGaa                                                                                        <  2:497491/139‑1 (MQ=255)
                              aaGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAAtatta                                                                                                                 >  1:204555/1‑108 (MQ=255)
                              aaGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAAtatta                                                                                                                 <  2:204555/108‑1 (MQ=255)
                              aaGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGc                                                                                                 >  2:551979/1‑124 (MQ=255)
                              aaGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGc                                                                                                 <  1:551979/124‑1 (MQ=255)
                                        gTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGAcc                                                                        <  1:229926/139‑1 (MQ=255)
                                                              cgcCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCa                                                  >  1:57708/1‑139 (MQ=255)
                                                                           gcgcGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCg                                                        <  1:769198/120‑1 (MQ=255)
                                                                           gcgcGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCg                                                        >  2:769198/1‑120 (MQ=255)
                                                                            cgcgGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTgcgc                                    <  2:90720/139‑1 (MQ=255)
                                                                                                      gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGAc          >  2:288634/1‑139 (MQ=255)
                                                                                                      gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGAc          >  2:293818/1‑139 (MQ=255)
                                                                                                                 gAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGat                                             >  1:628038/1‑93 (MQ=255)
                                                                                                                 gAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGat                                             <  2:628038/93‑1 (MQ=255)
                                                                                                                       cAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGt                                                                                     <  2:933096/47‑1 (MQ=255)
                                                                                                                       cAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGt                                                                                     >  1:933096/1‑47 (MQ=255)
                                                                                                                       cAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATc                                >  2:595783/1‑100 (MQ=255)
                                                                                                                       cAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATc                                <  1:595783/100‑1 (MQ=255)
                                                                                                                        aGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTggg                                                    >  2:357294/1‑79 (MQ=255)
                                                                                                                        aGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTggg                                                    <  1:357294/79‑1 (MQ=255)
                                                                                                                          tCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCg                                                        <  2:659/73‑1 (MQ=255)
                                                                                                                          tCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCg                                                        >  1:659/1‑73 (MQ=255)
                                                                                                                               cAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATcg  <  2:393642/122‑1 (MQ=255)
                                                                                                                               cAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATcg  >  1:393642/1‑122 (MQ=255)
                                                                                                                                  |                                                                                                                      
CGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCG  >  NC_000913/1606431‑1606679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: