Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	160,782	C→T	*235* (TAG→TAA)	sfsA ←	sugar fermentation stimulation protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	160,782	0	C	T	100.0%	58.5 / NA	20	*235* (TAG→TAA)	sfsA	sugar fermentation stimulation protein A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/10);  total (10/10)

AAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCA  >  NC_000913/160651‑160902                                                                                                                                    |                                                                                                                         aaaTGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATg                                                                                                                   >  2:468556/1‑139 (MQ=255)                      cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTaa                                                                                              >  2:354431/1‑139 (MQ=255)                                                 tAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCg                                                                   <  1:468556/139‑1 (MQ=255)                                                 aaggagaagcccTTGGCAACTGTGTTGAGAAAAGCGTAATTGGGCACGCGACCAGGATGGAAATTAACCAGTTAATTTCTTTATTACAATGTAACCGGCAGTTATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCg                                                                   <  1:586848/128‑1 (MQ=255)                                                                  aaCTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACa                                                                                                                      >  2:908507/1‑71 (MQ=255)                                                                  aaCTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACa                                                                                                                      <  1:908507/71‑1 (MQ=255)                                                                         tgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAccc                                           >  2:373622/1‑139 (MQ=255)                                                                           aGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttatta                                                                                                                        >  1:546367/1‑60 (MQ=255)                                                                           aGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAccccc                                         <  1:743695/139‑1 (MQ=255)                                                                                 aGCGTATTTGCGCACGCGACCAGAATGTAATTTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTACTACCCCCCTCTGt                                   >  2:190665/1‑139 (MQ=255)                                                                                        ttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCtt                            <  1:52670/139‑1 (MQ=255)                                                                                          gcgcACGCGGCCAGAATGTAAATTAACCAGTTACTTACTTTATTGCAATGTTGCCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAAGTTCCGCCGTGTAAGGCAGAATTTCTACCCCCCTCTGTTGAGCTTCt                          <  2:218098/139‑1 (MQ=255)                                                                                               cgcgACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcccccc                                        >  1:139191/1‑120 (MQ=255)                                                                                               cgcgACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcccccc                                        <  2:139191/120‑1 (MQ=255)                                                                                                          tGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGcgcg           <  1:930868/138‑1 (MQ=255)                                                                                                          tGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGcgcg           >  2:930868/1‑138 (MQ=255)                                                                                                                  aaCCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt                                                   >  1:720619/1‑90 (MQ=255)                                                                                                                  aaCCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt                                                   <  2:720619/90‑1 (MQ=255)                                                                                                                  aaCCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCa  >  1:451051/1‑139 (MQ=255)                                                                                                                    ccAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTc                                                                     <  2:291195/70‑1 (MQ=255)                                                                                                                    ccAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTc                                                                     >  1:291195/1‑70 (MQ=255)                                                                                                                                    |                                                                                                                         AAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCA  >  NC_000913/160651‑160902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑