| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 162,927 | G→A | A275T (GCT→ACT) | hrpB → | putative ATP‑dependent helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 162,927 | 0 | G | A | 100.0% | 110.5 / NA | 35 | A275T (GCT→ACT) | hrpB | putative ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/20); total (15/20) | |||||||||||
TTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCG > NC_000913/162803‑163038 | ttCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTa < 2:202952/139‑1 (MQ=255) tCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTaa < 2:451223/139‑1 (MQ=255) gTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc < 1:29839/139‑1 (MQ=255) gTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc < 2:387483/139‑1 (MQ=255) tGCCCGCTGTATGGCGCGTTGTCGCTGAAGGATCAGCGAAAAGCGTTCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggtgg > 2:536203/1‑139 (MQ=255) tGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggtgg > 2:732876/1‑139 (MQ=255) cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAgg < 1:410911/120‑1 (MQ=255) cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAgg > 2:410911/1‑120 (MQ=255) cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAt > 2:928634/1‑139 (MQ=255) cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAt > 1:454336/1‑139 (MQ=255) ccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt < 2:146244/139‑1 (MQ=255) ggCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACt > 1:240566/1‑87 (MQ=255) ggCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACt < 2:240566/87‑1 (MQ=255) tGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCgtgt > 2:726488/1‑139 (MQ=255) tCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGa < 1:250844/108‑1 (MQ=255) tCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGa > 2:250844/1‑108 (MQ=255) cAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATc < 1:726488/139‑1 (MQ=255) cGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACggg < 1:536203/139‑1 (MQ=255) cccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGTCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcg < 1:732876/139‑1 (MQ=255) cGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt < 2:454336/139‑1 (MQ=255) cGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTCGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt > 1:184208/1‑139 (MQ=255) cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGaaa < 2:325340/43‑1 (MQ=255) cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGaaa > 1:325340/1‑43 (MQ=255) cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGa < 2:933076/59‑1 (MQ=255) cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGa > 1:933076/1‑59 (MQ=255) ggATGCGCAAAGTGGTGCTGGCGACCAATACTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCa < 2:517048/139‑1 (MQ=255) gATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg < 1:482200/126‑1 (MQ=255) gATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg > 2:482200/1‑126 (MQ=255) gcgcAAAGTGGTGCTGGCGACCAATATTACTGaaa > 1:352684/1‑35 (MQ=37) gcgcAAAGTGGTGCTGGCGACCAATATTACTGaaa < 2:352684/35‑1 (MQ=255) gcgcAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc > 2:719569/1‑62 (MQ=255) gcgcAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc < 1:719569/62‑1 (MQ=255) cgcAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg < 1:928634/139‑1 (MQ=255) cAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc < 2:659992/41‑1 (MQ=255) cAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc > 1:659992/1‑41 (MQ=255) | TTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCG > NC_000913/162803‑163038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |