Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,719,058	G→A	P182L (CCG→CTG)	anmK ←	anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,719,058	0	G	A	100.0%	72.8 / NA	25	P182L (CCG→CTG)	anmK	anhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/10);  total (15/10)

CAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCA  >  NC_000913/1718931‑1719156                                                                                                                                |                                                                                                   cagcagTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGa                                                                                         >  1:179351/1‑139 (MQ=255)    cagTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTACCGTAGCCCCCAACCAGCTGCCCAGGAATg                                                                                      <  1:292190/139‑1 (MQ=255)     agTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGcc                                                                                              >  1:261715/1‑130 (MQ=255)     agTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGcc                                                                                              <  2:261715/130‑1 (MQ=255)            gaATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGa                                                                              <  2:136256/139‑1 (MQ=255)                  cattACCCCCCCGTGCCCACTCGGAAGCGTTATCGTAAGTTTTACCGGCATGACGCCACATCCAGGCATCCCTCAGCCTGATACCAGGACCGGTATCATAGCCCCCAACCAGCTGCCCAGGAATGACCAGTGACAGAtt                                                                        >  2:442199/3‑139 (MQ=255)                  cTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACTAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGAtt                                                                        >  1:422665/1‑139 (MQ=255)                    ttACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTgg                                                                      >  1:174628/1‑139 (MQ=255)                       cccgcccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGa                                                                   >  1:343663/1‑139 (MQ=255)                          gcccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGAAGTGACAGATTGGCGATgc                                                                >  2:608766/1‑139 (MQ=255)                           cccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATgcc                                                               >  2:335070/1‑139 (MQ=255)                             cgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATgccgc                                                             <  2:343663/139‑1 (MQ=255)                                    aCTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATAtt                                                      <  2:174628/139‑1 (MQ=255)                                                        ggTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCgg                                  <  1:335070/139‑1 (MQ=255)                                                             aCCGGCCTGTCGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACTGGTATCGTAGCCCCCAAGCAGCTGCTCAGGAATGAGCAGAGACAGATTGCCGTTGCGGCCAATATTGAGCACCATTCGACGCTGGATTggt                             >  2:438568/1‑138 (MQ=255)                                                             aCCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGcc                                                                                              >  1:919508/1‑74 (MQ=255)                                                             aCCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGcc                                                                                              <  2:919508/74‑1 (MQ=255)                                                                       cGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCcagca                   <  1:464602/139‑1 (MQ=255)                                                                             aTCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGATAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCat             >  1:229174/1‑139 (MQ=255)                                                                                cAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCatgat          <  2:179351/139‑1 (MQ=255)                                                                                    catccatcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGaa      >  2:2906/1‑139 (MQ=255)                                                                                        catcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa  >  2:607104/1‑139 (MQ=255)                                                                                        catcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa  >  2:797385/1‑139 (MQ=255)                                                                                                                         cccAACCAGCTGCCCAGGAATGAGCAGTGGCAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa  <  2:666912/106‑1 (MQ=255)                                                                                                                         cccAACCAGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCa  >  1:666912/1‑106 (MQ=255)                                                                                                                                |                                                                                                   CAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCA  >  NC_000913/1718931‑1719156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑