Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,745,362	T→C	T256A (ACA→GCA)	ydhQ ←	autotransporter adhesin‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,745,362	0	T	C	100.0%	174.8 / NA	48	T256A (ACA→GCA)	ydhQ	autotransporter adhesin‑related protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/24);  total (24/24)

CAGCTCTGCGCCATAACCAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGTCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACGATAAATGGAT  >  NC_000913/1745228‑1745495                                                                                                                                       |                                                                                                                                      cAGCTCTGCGCCATAACCAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCa                                                                                                                                   <  2:123905/139‑1 (MQ=255)      ctGCGCCATAACCAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTg                                                                                                                              <  1:809880/139‑1 (MQ=255)         cgcCATAACCAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTAt                                                                                                                           >  1:105576/1‑139 (MQ=255)                  cAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGtt                                                                                                                  <  1:413950/139‑1 (MQ=255)                       atCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCa                                                                                                             <  2:68275/139‑1 (MQ=255)                            tGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCa                                                                                                             <  2:779769/134‑1 (MQ=255)                            tGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCa                                                                                                             >  1:779769/1‑134 (MQ=255)                                      tCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCATCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCa                                                                                              >  2:634023/1‑139 (MQ=255)                                        cAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACt                                                                                            >  1:862787/1‑139 (MQ=255)                                              tGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGcc                                                                                      <  2:105576/139‑1 (MQ=255)                                                cAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCg                                                                                    >  2:62828/1‑139 (MQ=255)                                                cAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGAATGCACTTCAGCCCg                                                                                    >  2:477798/1‑139 (MQ=255)                                                 aaCACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGa                                                                                   <  1:477798/139‑1 (MQ=255)                                                     cTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGaccac                                                                               >  2:240903/1‑139 (MQ=255)                                                        ccGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGac                                                                                  <  2:328930/133‑1 (MQ=255)                                                        ccGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGac                                                                                  >  1:328930/1‑133 (MQ=255)                                                           gTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACcgcg                                                                                                                    <  1:329628/96‑1 (MQ=255)                                                           gTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACcgcg                                                                                                                    >  2:329628/1‑96 (MQ=255)                                                                  cAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGa                                                                  <  1:240903/139‑1 (MQ=255)                                                                         tGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGttt                                                           >  2:453433/1‑139 (MQ=255)                                                                          gcgcGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGtttt                                                          >  2:279571/1‑139 (MQ=255)                                                                                          cGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACt                                          >  2:290214/1‑139 (MQ=255)                                                                                          cGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACt                                          >  2:62262/1‑139 (MQ=255)                                                                                          cGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACt                                          >  2:98559/1‑139 (MQ=255)                                                                                          cGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACt                                          >  1:125691/1‑139 (MQ=255)                                                                                          cGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACt                                          >  1:325479/1‑139 (MQ=255)                                                                                           gACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTg                                         <  2:325479/139‑1 (MQ=255)                                                                                            aCAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGCACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGt                                        >  1:49085/1‑139 (MQ=255)                                                                                                          cgcgCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCa                          <  1:98559/139‑1 (MQ=255)                                                                                                           gcgcTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCAt                         <  1:290214/139‑1 (MQ=255)                                                                                                            cgcTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATc                        <  2:125691/139‑1 (MQ=255)                                                                                                            cgcTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATc                        <  1:62262/139‑1 (MQ=255)                                                                                                                  tgggtgACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTccc                  <  1:453433/139‑1 (MQ=255)                                                                                                                       gACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGAc             >  1:659121/1‑139 (MQ=255)                                                                                                                        acaacaGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACg            <  1:341761/139‑1 (MQ=255)                                                                                                                           acaGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGc                            <  2:293213/120‑1 (MQ=255)                                                                                                                           acaGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGc                            >  1:293213/1‑120 (MQ=255)                                                                                                                            caGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGaa                                            <  1:461421/103‑1 (MQ=255)                                                                                                                            caGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGaa                                            >  2:461421/1‑103 (MQ=255)                                                                                                                               gATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGaccacc                                                                              <  1:914041/66‑1 (MQ=255)                                                                                                                               gATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGaccacc                                                                              >  2:914041/1‑66 (MQ=255)                                                                                                                               gATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCgg                                              >  1:303640/1‑98 (MQ=255)                                                                                                                               gATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCgg                                              <  2:303640/98‑1 (MQ=255)                                                                                                                               gATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACGATAAATg     >  2:431175/1‑139 (MQ=255)                                                                                                                                  cACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACGATAAATGGAt  <  1:62828/139‑1 (MQ=255)                                                                                                                                  cACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGCACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACGATAAATGGAt  <  2:49085/139‑1 (MQ=255)                                                                                                                                    cTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGAccaccca                                                                            <  1:121160/63‑1 (MQ=255)                                                                                                                                    cTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGAccaccca                                                                            >  2:121160/1‑63 (MQ=255)                                                                                                                                       |                                                                                                                                      CAGCTCTGCGCCATAACCAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGTCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACGATAAATGGAT  >  NC_000913/1745228‑1745495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑