Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,764,018 C→T *123* (TAG→TAA sufA ← Fe‑S cluster assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,764,0180CT100.0% 91.2 / NA 30*123* (TAG→TAAsufAFe‑S cluster assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/14);  total (16/14)

TCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGCGGG  >  NC_000913/1763898‑1764150
                                                                                                                        |                                                                                                                                    
tCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgc                                                                                                                   >  1:341300/1‑139 (MQ=255)
      ttGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                             <  1:916048/139‑1 (MQ=255)
           aGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCAcat                                                                                                        <  2:341300/139‑1 (MQ=255)
                   ggTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                                <  2:714904/139‑1 (MQ=255)
                               ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTa                                                                                         >  1:72250/1‑134 (MQ=255)
                               ggTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTa                                                                                         <  2:72250/134‑1 (MQ=255)
                                          cttcttTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGa                                                                         <  1:809550/139‑1 (MQ=255)
                                                       cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACattcattc                                                                                                  >  1:218697/1‑101 (MQ=255)
                                                       cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACattcattc                                                                                                  <  2:218697/101‑1 (MQ=255)
                                                       cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                                >  2:737938/1‑103 (MQ=255)
                                                       cAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                                <  1:737938/103‑1 (MQ=255)
                                                        aGCGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGt                                                           >  1:33774/1‑139 (MQ=255)
                                                          cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                             <  2:582630/87‑1 (MQ=255)
                                                          cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                             <  2:467994/87‑1 (MQ=255)
                                                          cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                             >  1:582630/1‑87 (MQ=255)
                                                          cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccacagc                                                                                                             >  1:467994/1‑87 (MQ=255)
                                                          cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccaca                                                                                                               <  1:285316/85‑1 (MQ=255)
                                                          cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTcc                                                         >  1:242577/1‑139 (MQ=255)
                                                          cGGGCCGCCGGTCCAGGTTTTGACATCGTCATTGCTACAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCgccaca                                                                                                               >  2:285316/1‑85 (MQ=255)
                                                                    gTCCAGGTTTTGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCacgaacg                                               <  2:242577/139‑1 (MQ=255)
                                                                            tttGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCa                                                                                                                             >  2:10739/1‑53 (MQ=255)
                                                                            tttGACATCGTCATTGCTTCAGTATTACGAGACATAGTACCGCTTATACCCCa                                                                                                                             <  1:10739/53‑1 (MQ=255)
                                                                                                 aGTATTACGAGACATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCACAATAAACgg                  >  1:151481/1‑139 (MQ=255)
                                                                                                           gaCATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTg         >  2:769258/1‑139 (MQ=255)
                                                                                                             cATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                                <  1:754529/50‑1 (MQ=255)
                                                                                                             cATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTg                                                                                                >  2:754529/1‑50 (MQ=255)
                                                                                                             cATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGc                 >  2:267936/1‑129 (MQ=255)
                                                                                                             cATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGc                 <  1:267936/129‑1 (MQ=255)
                                                                                                             cATAGTACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCa       >  2:574082/1‑139 (MQ=255)
                                                                                                                  tACCGCTTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGCggg  >  2:812032/1‑139 (MQ=255)
                                                                                                                        |                                                                                                                                    
TCCCCTTTGCCAGCTCATCGGTGGCTAACTGGGTGAAGAATCCTTCTTTATAATTCAGCGGGCCGCCGGTCCAGGTTTTGACATCGTCAGTTGCTTCAGTATTACGAGACATAGTACCGCCTATACCCCAAAGCTTTCGCCACAGCCACATTCATTCTGGGCTTTAGGGTTGTGAAATTTGAATATCTGATTAAGTCCTTCACGAACGAAATCGACTTCCGTGCCATCAATAAACGGCATCGCTTGCAGCGGG  >  NC_000913/1763898‑1764150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: