Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	177,662	C→T	*267* (TAG→TAA)	btuF ←	vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	177,662	0	C	T	100.0%	153.6 / NA	49	*267* (TAG→TAA)	btuF	vitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/23);  total (26/23)

CACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGCTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCC  >  NC_000913/177531‑177780                                                                                                                                    |                                                                                                                       cacCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTa                                                                                                                 >  2:803213/1‑139 (MQ=255)              cATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAgcgc                                                                                                    >  2:560632/1‑139 (MQ=255)              cATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAgcgc                                                                                                    >  1:468918/1‑139 (MQ=255)                     aaTTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACag                                                                                             <  2:468918/139‑1 (MQ=255)                                 aaCAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCa                                                                                 <  1:44347/139‑1 (MQ=255)                                  aCAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAg                                                                                <  1:162481/139‑1 (MQ=255)                                    aaaaCGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCg                                                                              <  1:560632/139‑1 (MQ=255)                                    aaaaCGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCg                                                                              <  1:803213/139‑1 (MQ=255)                                                             cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAgcg                                                                                                     >  1:237104/1‑91 (MQ=255)                                                             cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAgcg                                                                                                     <  2:237104/91‑1 (MQ=255)                                                             cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGtt                                                     >  2:64480/1‑139 (MQ=255)                                                             cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGtt                                                     >  2:361784/1‑139 (MQ=255)                                                             cTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGtt                                                     >  2:237731/1‑139 (MQ=255)                                                                        atatCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCa                                          <  1:237731/139‑1 (MQ=255)                                                                          atCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                           <  1:166114/88‑1 (MQ=255)                                                                          atCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                           >  2:166114/1‑88 (MQ=255)                                                                               gCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCaaa                                                 >  2:918332/1‑125 (MQ=255)                                                                               gCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCaaa                                                 <  1:918332/125‑1 (MQ=255)                                                                                ccAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGt                                            <  1:601619/129‑1 (MQ=255)                                                                                ccAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGt                                            >  2:601619/1‑129 (MQ=255)                                                                                 cAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGCAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTgaga                                 >  2:634965/1‑139 (MQ=255)                                                                                 cAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTgaga                                 >  2:520459/1‑139 (MQ=255)                                                                                   aTAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGc                                                          >  1:664042/1‑112 (MQ=255)                                                                                   aTAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGc                                                          <  2:664042/112‑1 (MQ=255)                                                                                   aTAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGc                                                          <  2:221301/112‑1 (MQ=255)                                                                                   aTAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGc                                                          >  1:221301/1‑112 (MQ=255)                                                                                                         ccACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGc                                                                               >  1:16213/1‑69 (MQ=255)                                                                                                         ccACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGc                                                                               <  2:16213/69‑1 (MQ=255)                                                                                                         ccACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGt         >  1:600313/1‑139 (MQ=255)                                                                                                          cACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGtt        <  1:64480/139‑1 (MQ=255)                                                                                                          cACTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGtt        <  1:520459/139‑1 (MQ=255)                                                                                                           aCTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTc                                                    <  1:602728/94‑1 (MQ=255)                                                                                                           aCTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTc                                                    >  2:602728/1‑94 (MQ=255)                                                                                                           aCTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTc                                       <  1:385433/107‑1 (MQ=255)                                                                                                           aCTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTc       >  2:38537/1‑139 (MQ=255)                                                                                                           aCTGAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCAGTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTc                                       >  2:385433/1‑107 (MQ=255)                                                                                                              gAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCAcc    >  1:279035/1‑139 (MQ=255)                                                                                                              gAGCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCAcc    <  1:634965/139‑1 (MQ=255)                                                                                                                gCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCAt                                                                                                  <  1:654930/43‑1 (MQ=255)                                                                                                                gCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCAt                                                                                                  >  2:654930/1‑43 (MQ=255)                                                                                                                gCACCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCAcccc  >  1:847437/1‑139 (MQ=255)                                                                                                                  aCCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                           >  2:133564/1‑48 (MQ=255)                                                                                                                  aCCTGAAAAGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACagag                                                                                           <  1:133564/48‑1 (MQ=255)                                                                                                                         aaGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCAccc   <  2:74344/129‑1 (MQ=255)                                                                                                                         aaGGTCAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCAccc   >  1:74344/1‑129 (MQ=255)                                                                                                                              cAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGa                                                                          <  1:336309/53‑1 (MQ=255)                                                                                                                              cAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGa                                                                          >  2:336309/1‑53 (MQ=255)                                                                                                                              cAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAg             <  1:52785/114‑1 (MQ=255)                                                                                                                              cAGGCGTTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAg             >  2:52785/1‑114 (MQ=255)                                                                                                                                    |                                                                                                                       CACCAAAAGGGTCCATACGCAATTTTCCGGCTAACAAAACGCCGGAGATGGCAAATACCGCTGTGCCGACTATATCCAGCCAATAGACGAGCATTGTTCAATCCCCACTGAGCACCTGAAAAGGTCAGGCGCTAATCTACCTGTGAAAGCGCATTACAGAGCTGTTGTGCAGCGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCC  >  NC_000913/177531‑177780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑