Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,842,910	G→A	A180A (GCG→GCA)	gdhA →	glutamate dehydrogenase, NADP‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,842,910	0	G	A	100.0%	59.4 / NA	20	A180A (GCG→GCA)	gdhA	glutamate dehydrogenase, NADP‑specific
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/11);  total (9/11)

AGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAG  >  NC_000913/1842790‑1843031                                                                                                                         |                                                                                                                          aGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc                                                                                                         <  2:154841/139‑1 (MQ=255) aGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc                                                                                                         <  2:339067/139‑1 (MQ=255)                                        aCTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCtt                                                                  >  2:330564/1‑139 (MQ=255)                                            tATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCAt                                                              >  1:832476/1‑139 (MQ=255)                                                    ccTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc                                                                                                         >  1:688655/1‑88 (MQ=255)                                                    ccTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc                                                                                                         <  2:688655/88‑1 (MQ=255)                                                          cgcgGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCtt                                                >  1:525519/1‑139 (MQ=255)                                                              gATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTc                                            >  1:471933/1‑139 (MQ=255)                                                                tACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc                                                                                                         <  1:386557/76‑1 (MQ=255)                                                                tACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc                                                                                                         >  2:386557/1‑76 (MQ=255)                                                                 aCCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc                                                                                                         >  1:832013/1‑75 (MQ=255)                                                                 aCCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGctc                                                                                                         <  2:832013/75‑1 (MQ=255)                                                                         tCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCt                                 >  2:235619/1‑139 (MQ=255)                                                                          ccGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTa                                <  1:235619/139‑1 (MQ=255)                                                                                           ggTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCg                                                                                      >  1:354688/1‑68 (MQ=255)                                                                                           ggTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCg                                                                                      <  2:354688/68‑1 (MQ=255)                                                                                           ggTTGGTGGTCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGtt               <  2:839323/139‑1 (MQ=255)                                                                                                    tCGTGAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTcaca      <  2:832476/139‑1 (MQ=255)                                                                                                        gAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAg  <  2:414304/139‑1 (MQ=255)                                                                                                        gAAGTCGGCTTTATGGCAGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAg  <  2:36120/139‑1 (MQ=255)                                                                                                                         |                                                                                                                          AGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACCGGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAG  >  NC_000913/1842790‑1843031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑