Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,881,463	G→A	A116V (GCG→GTG)	dmlR ←	transcriptional activator of dmlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,881,463	0	G	A	100.0%	158.8 / NA	50	A116V (GCG→GTG)	dmlR	transcriptional activator of dmlA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/24);  total (26/24)

TTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCG  >  NC_000913/1881330‑1881595                                                                                                                                      |                                                                                                                                     tttGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACtaat                                                                                                                                 >  1:886196/1‑139 (MQ=255)       ggCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGcc                                                                                                                           <  1:646771/139‑1 (MQ=255)       ggCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCAATAATAACGcc                                                                                                                           >  2:117340/1‑139 (MQ=255)        gCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACCTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAacgacg                                                                                                                          <  2:538461/139‑1 (MQ=255)         cGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCgg                                                                                                                         <  2:646941/139‑1 (MQ=255)           aTTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAg                                                                                                                       <  2:16561/139‑1 (MQ=255)           aTTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAg                                                                                                                       >  2:170533/1‑139 (MQ=255)                     gggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTg                                                                                                             >  2:633065/1‑139 (MQ=255)                     gggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTg                                                                                                             >  1:539879/1‑139 (MQ=255)                                       tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                                                           <  1:477289/139‑1 (MQ=255)                                       tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                                                           <  2:100900/139‑1 (MQ=255)                                                      cGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACgcag                                                                            >  1:831327/1‑139 (MQ=255)                                                        acacCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAAc                                                                                                                              <  1:430318/87‑1 (MQ=255)                                                        acacCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAAc                                                                                                                              >  2:430318/1‑87 (MQ=255)                                                        acacCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACgcagc                                                                           >  2:172816/1‑138 (MQ=255)                                                        acacCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACgcagc                                                                           <  1:172816/138‑1 (MQ=255)                                                         cacCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCAt                                                                         >  1:358763/1‑139 (MQ=255)                                                         cacCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACGAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCCGCTGATAATACGCAGCAt                                                                         >  1:384428/1‑139 (MQ=255)                                                           ccTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGataata                                                                                 >  1:154974/1‑129 (MQ=255)                                                           ccTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGataata                                                                                 <  2:154974/129‑1 (MQ=255)                                                                 ttGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCAATAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCg                                                                 <  1:117340/139‑1 (MQ=255)                                                                  tGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCgg                                                                <  1:172800/139‑1 (MQ=255)                                                                   gACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAAc                                                                                                                              <  2:558332/76‑1 (MQ=255)                                                                   gACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAAc                                                                                                                              >  1:558332/1‑76 (MQ=255)                                                                    aCCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCa                                                              >  1:877768/1‑139 (MQ=255)                                                                      cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCAcc                                                            >  1:44932/1‑139 (MQ=255)                                                                      cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTACGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACgca                                                                             <  1:184639/122‑1 (MQ=255)                                                                      cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTACGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACgca                                                                             >  2:184639/1‑122 (MQ=255)                                                                              ccAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTACCAACGCCGGCGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCAc                                                    >  1:499608/1‑139 (MQ=255)                                                                                         tCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACg                                                       >  1:773600/1‑125 (MQ=255)                                                                                         tCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACg                                                       <  2:773600/125‑1 (MQ=255)                                                                                         tCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGtt                                         <  2:877768/139‑1 (MQ=255)                                                                                              aTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCAt                                                                         >  1:603551/1‑102 (MQ=255)                                                                                              aTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCGGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCAt                                                                         <  2:603551/102‑1 (MQ=255)                                                                                                cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCcat                                  >  1:573132/1‑139 (MQ=255)                                                                                                cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCcat                                  >  1:201063/1‑139 (MQ=255)                                                                                                cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCcat                                  >  1:647235/1‑139 (MQ=255)                                                                                                        aaTTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGAc                          <  2:828562/139‑1 (MQ=255)                                                                                                            ccAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGAc                      <  2:499608/139‑1 (MQ=255)                                                                                                               gTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACTAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCa                                                     <  2:659265/105‑1 (MQ=255)                                                                                                               gTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGataata                                                                                 <  1:555990/77‑1 (MQ=255)                                                                                                               gTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGataata                                                                                 >  2:555990/1‑77 (MQ=255)                                                                                                               gTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCa                                                     >  1:659265/1‑105 (MQ=255)                                                                                                                  gCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgctgc                                                                                         >  2:909461/1‑66 (MQ=255)                                                                                                                  gCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgctgc                                                                                         <  1:909461/66‑1 (MQ=255)                                                                                                                     gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgctgc                                                                                         >  2:655748/1‑63 (MQ=255)                                                                                                                     gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgctgc                                                                                         <  1:655748/63‑1 (MQ=255)                                                                                                                                gCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTgcg  <  2:647235/139‑1 (MQ=255)                                                                                                                                gCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTgcg  <  2:201063/139‑1 (MQ=255)                                                                                                                                gCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTgcg  >  2:186275/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                     TTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCG  >  NC_000913/1881330‑1881595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑