Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,910,474	T→C	S67P (TCC→CCC)	yebQ →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,910,474	0	T	C	100.0%	118.3 / NA	34	S67P (TCC→CCC)	yebQ	putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/19);  total (15/19)

TGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTG  >  NC_000913/1910344‑1910609                                                                                                                                   |                                                                                                                                        tGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTACCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGctc                                                                                                                                 <  2:903227/139‑1 (MQ=255)  ggTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGctct                                                                                                                                >  1:118314/1‑139 (MQ=255)                  tCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTggg                                                                                                                >  2:42894/1‑139 (MQ=255)                    cTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                              <  1:496934/139‑1 (MQ=255)                                   gCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATc                                                                                               <  2:520122/139‑1 (MQ=255)                                     cAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGa                                                                                             <  1:222052/139‑1 (MQ=255)                                     cAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGa                                                                                             <  1:42894/139‑1 (MQ=255)                                         gTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTa                                                                                         <  1:443742/139‑1 (MQ=255)                                                                       cATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTTTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgt                                                           <  2:172929/139‑1 (MQ=255)                                                                       cATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgt                                                           >  2:270973/1‑139 (MQ=255)                                                                        aTGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgtt                                                          <  1:213753/139‑1 (MQ=255)                                                                            cACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCt                                                      >  1:698183/1‑139 (MQ=255)                                                                              cGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATg                                                                               <  1:125711/112‑1 (MQ=255)                                                                              cGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATg                                                                               >  2:125711/1‑112 (MQ=255)                                                                              cGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTc                                                    >  1:850581/1‑139 (MQ=255)                                                                               gccagccaGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCa                                                   <  2:850581/139‑1 (MQ=255)                                                                                    ccagTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGtt                                              >  1:138250/1‑139 (MQ=255)                                                                                     cagTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTc                                             <  1:386461/139‑1 (MQ=255)                                                                                         tCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTgcg                                         >  1:897452/1‑139 (MQ=255)                                                                                         tCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTgcg                                         <  2:291256/139‑1 (MQ=255)                                                                                           cATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGcc                                       <  1:544013/139‑1 (MQ=255)                                                                                             tCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCt                                     >  1:746860/1‑139 (MQ=255)                                                                                                 ggTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgttgt                                                        >  1:842410/1‑116 (MQ=255)                                                                                                 ggTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgttgt                                                        <  2:842410/116‑1 (MQ=255)                                                                                                  gTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTg                                >  2:10548/1‑139 (MQ=255)                                                                                                        aaCGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTg                                           >  2:193819/1‑122 (MQ=255)                                                                                                        aaCGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTg                                           <  1:193819/122‑1 (MQ=255)                                                                                                             cTATCAAATCGCCCTTCTCATCCCCCTCCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCaa                     <  2:897452/139‑1 (MQ=255)                                                                                                             cTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCaa                     >  2:104019/1‑139 (MQ=255)                                                                                                                tCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                              <  1:191459/47‑1 (MQ=255)                                                                                                                tCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                              >  2:191459/1‑47 (MQ=255)                                                                                                                tCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCg                                                                                              <  1:2826/63‑1 (MQ=255)                                                                                                                tCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCg                                                                                              >  2:2826/1‑63 (MQ=255)                                                                                                                                aTCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCgtg  <  2:698183/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        TGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTG  >  NC_000913/1910344‑1910609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑