Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,022,606	G→A	*246* (TAG→TAA)	fliP →	flagellar biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,022,606	0	G	A	100.0%	67.7 / NA	22	*246* (TAG→TAA)	fliP	flagellar biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/11);  total (11/11)

GCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCAC  >  NC_000913/2022473‑2022740                                                                                                                                      |                                                                                                                                       gCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAgg                                                                                                                                   >  1:357918/1‑139 (MQ=255) gCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAgg                                                                                                                                   >  2:768244/1‑139 (MQ=255)  cGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGc                                                                                                                                  >  2:378208/1‑139 (MQ=255)                 ggggATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGaa                                                                                                                   <  1:378208/139‑1 (MQ=255)                                  ccccAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATggg                                                                                                  >  1:829854/1‑139 (MQ=255)                                  ccccAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATggg                                                                                                  >  2:307917/1‑139 (MQ=255)                                   cccAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGGCACCTGAATCGGTCATGATGATgggg                                                                                                 >  1:568063/1‑139 (MQ=255)                                          accaTTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGt                                                                                                              <  1:772757/119‑1 (MQ=255)                                          accaTTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGt                                                                                                              >  2:772757/1‑119 (MQ=255)                                                                tGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGa                                                                                                <  1:552653/111‑1 (MQ=255)                                                                tGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGa                                                                                                >  2:552653/1‑111 (MQ=255)                                                                     cTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTg                                                               <  2:829854/139‑1 (MQ=255)                                                                      tGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGGCACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGc                                                              <  2:568063/139‑1 (MQ=255)                                                                          tGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCg                                                          <  1:681916/139‑1 (MQ=255)                                                                                ggtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTATAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGccc                                                            >  1:599438/1‑131 (MQ=255)                                                                                ggtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTATAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGccc                                                            <  2:599438/131‑1 (MQ=255)                                                                                ggtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgcg                                                                            <  1:33588/115‑1 (MQ=255)                                                                                ggtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTcgcg                                                                            >  2:33588/1‑115 (MQ=255)                                                                                                ttGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCa                                    <  1:411164/139‑1 (MQ=255)                                                                                                                        agCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGACACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGc            >  1:650566/1‑139 (MQ=255)                                                                                                                         gCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCa           <  1:768244/139‑1 (MQ=255)                                                                                                                                  gCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCAc  <  2:496784/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       GCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCAC  >  NC_000913/2022473‑2022740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑