Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,059,964	C→T	*317* (TAG→TAA)	cbl ←	ssuEADCB/tauABCD operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,059,964	0	C	T	100.0%	107.9 / NA	34	*317* (TAG→TAA)	cbl	ssuEADCB/tauABCD operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

ACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAGT  >  NC_000913/2059846‑2060096                                                                                                                       |                                                                                                                                     acGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTc                                                                                                                  >  1:691022/1‑139 (MQ=255)           tGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                        <  1:928838/139‑1 (MQ=255)           tGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                        <  2:691022/139‑1 (MQ=255)           tGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                        <  1:261085/139‑1 (MQ=255)                ttCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTc                                                                                                   >  1:184474/1‑139 (MQ=255)                     tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCa                                                                                              >  2:586504/1‑139 (MQ=255)                                   gACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGata                                                                                >  2:521461/1‑139 (MQ=255)                                    aCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGatat                                                                               <  1:521461/139‑1 (MQ=255)                                         tAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCa                                                                          >  2:386324/1‑139 (MQ=255)                                                tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACa                                                                   >  1:39387/1‑139 (MQ=255)                                                      aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTg                                                             >  2:740100/1‑139 (MQ=255)                                                        tGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCa                                                           <  1:586504/139‑1 (MQ=255)                                                          gTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAg                                                                  <  1:346502/130‑1 (MQ=255)                                                          gTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAg                                                                  >  2:346502/1‑130 (MQ=255)                                                          gTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCAtt                                                         >  2:226188/1‑139 (MQ=255)                                                             cGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                                      <  1:31130/139‑1 (MQ=255)                                                              gAGTCCAGTCAGAGGCGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTCCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                                >  2:620504/1‑128 (MQ=255)                                                              gAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                                <  1:620504/128‑1 (MQ=255)                                                                    aGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTcc                                               >  1:272222/1‑139 (MQ=255)                                                                       cAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGa                                            <  1:740100/139‑1 (MQ=255)                                                                            gagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcg                                       <  1:386324/139‑1 (MQ=255)                                                                               ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACt                                                                                                         <  2:29668/70‑1 (MQ=255)                                                                               ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACt                                                                                                         >  1:29668/1‑70 (MQ=255)                                                                               ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGc                                                                                                       >  2:103155/1‑72 (MQ=255)                                                                               ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGc                                                                                                       <  1:103155/72‑1 (MQ=255)                                                                               ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGc                                                                                                       <  2:304320/72‑1 (MQ=255)                                                                               ccAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGc                                                                                                       >  1:304320/1‑72 (MQ=255)                                                                                   aTTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAAt                                                                                                                     >  2:909222/1‑54 (MQ=255)                                                                                   aTTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAAt                                                                                                                     <  1:909222/54‑1 (MQ=255)                                                                                      ccTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAg                             <  2:184474/139‑1 (MQ=255)                                                                                       cTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGatat                                                                               >  1:841992/1‑88 (MQ=255)                                                                                       cTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGatat                                                                               <  2:841992/88‑1 (MQ=255)                                                                                                        ataGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCt           <  1:226188/139‑1 (MQ=255)                                                                                                                 tttttGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAGt  <  1:737335/139‑1 (MQ=255)                                                                                                                       |                                                                                                                                     ACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTCGCTTCAGT  >  NC_000913/2059846‑2060096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑