Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,090,046	G→A	*17* (TAG→TAA)	hisL →	his operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,090,046	0	G	A	100.0%	174.5 / NA	55	*17* (TAG→TAA)	hisL	his operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/26);  total (29/26)

AATATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGA  >  NC_000913/2089911‑2090180                                                                                                                                        |                                                                                                                                       aaTATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCt                                                                                                                                     >  2:37492/1‑139 (MQ=255)     tataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCa                                                                                                                                 >  1:559261/1‑139 (MQ=255)        aaaaaaGCCCTTGCTTTCTAACGTGAAAGGGGGTTAGGTTAAAAGTCATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCAACCTTACTAATCTTTCAAGc                                                                                                                              >  2:911281/1‑139 (MQ=255)            aaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAACCACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg                                                                                                                          <  2:658156/139‑1 (MQ=255)             aGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgt                                                                                                                         >  1:542778/1‑139 (MQ=255)             aGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           <  2:331076/137‑1 (MQ=255)             aGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           >  1:331076/1‑137 (MQ=255)               cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgt                                                                                                                       >  1:682109/1‑139 (MQ=255)               cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtg                                                                                                                        >  2:394148/1‑138 (MQ=255)               cccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtg                                                                                                                        <  1:394148/138‑1 (MQ=255)                          tAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               <  1:83504/136‑1 (MQ=255)                          tAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               >  2:83504/1‑136 (MQ=255)                                            ggTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTcc                                                                                                >  2:266597/1‑133 (MQ=255)                                            ggTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTcc                                                                                                <  1:266597/133‑1 (MQ=255)                                                       aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCTCCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              >  1:68542/1‑124 (MQ=255)                                                       aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCTCCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              <  2:68542/124‑1 (MQ=255)                                                        tCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCAt                                                                              >  2:134869/1‑139 (MQ=255)                                                            ttGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCGTGAACGCATgaga                                                                          <  1:37492/139‑1 (MQ=255)                                                            ttGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATgaga                                                                          <  1:668896/139‑1 (MQ=255)                                                                   aaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGATTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGcccc                                                                   <  2:554005/139‑1 (MQ=255)                                                                      aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCa                                                                                                        >  1:438657/1‑99 (MQ=255)                                                                      aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCa                                                                                                        <  2:438657/99‑1 (MQ=255)                                                                      aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTcc                                                                                                <  1:703691/107‑1 (MQ=255)                                                                      aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTcc                                                                                                >  2:703691/1‑107 (MQ=255)                                                                           caGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGAt                                                           <  1:807158/139‑1 (MQ=255)                                                                              agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               <  1:678183/68‑1 (MQ=255)                                                                              agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               >  2:678183/1‑68 (MQ=255)                                                                              agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  2:306722/83‑1 (MQ=255)                                                                              agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                >  1:306722/1‑83 (MQ=255)                                                                                    ttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                >  1:141761/1‑77 (MQ=255)                                                                                    ttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  2:141761/77‑1 (MQ=255)                                                                                          cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                >  2:805427/1‑71 (MQ=255)                                                                                          cacGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  1:805427/71‑1 (MQ=255)                                                                                            cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg                                                                                                                          >  1:619449/1‑59 (MQ=255)                                                                                            cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg                                                                                                                          <  2:619449/59‑1 (MQ=255)                                                                                            cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGAt                                                           >  2:331030/1‑122 (MQ=255)                                                                                            cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGAt                                                           <  1:331030/122‑1 (MQ=255)                                                                                                       tAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGcgcg                               <  1:225206/139‑1 (MQ=255)                                                                                                               accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  2:924782/50‑1 (MQ=255)                                                                                                               accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                >  1:924782/1‑50 (MQ=255)                                                                                                               accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGccccc                                                                  >  2:303331/1‑96 (MQ=255)                                                                                                               accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGccccc                                                                  <  1:303331/96‑1 (MQ=255)                                                                                                               accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                       >  1:750644/1‑139 (MQ=255)                                                                                                               accatcatcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                       >  1:326791/1‑139 (MQ=255)                                                                                                                           atcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                >  1:679230/1‑38 (MQ=255)                                                                                                                           atcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  2:679230/38‑1 (MQ=255)                                                                                                                             catcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              >  1:158729/1‑54 (MQ=255)                                                                                                                             catcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              <  2:158729/54‑1 (MQ=255)                                                                                                                               tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAg                                                                       <  2:493676/75‑1 (MQ=255)                                                                                                                               tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAg                                                                       >  1:493676/1‑75 (MQ=255)                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      <  1:888551/39‑1 (MQ=255)                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      >  2:888551/1‑39 (MQ=255)                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              >  2:264092/1‑47 (MQ=255)                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              <  1:264092/47‑1 (MQ=255)                                                                                                                                    aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga  >  2:640015/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       AATATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGA  >  NC_000913/2089911‑2090180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑