Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,104,494 C→T V6I (GTC→ATC) 
*197* (TAG→TAA
wbbK ←
wbbJ ←
lipopolysaccharide biosynthesis protein
putative lipopolysaccharide biosynthesis O‑acetyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,104,4940CT100.0% 93.4 / NA 30V6I (GTC→ATC) 
*197* (TAG→TAA
wbbK
wbbJ
lipopolysaccharide biosynthesis protein
putative lipopolysaccharide biosynthesis O‑acetyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/17);  total (13/17)

ACTTTCTTTTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGACTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGA  >  NC_000913/2104360‑2104615
                                                                                                                                      |                                                                                                                         
aCTTTCTTTTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATg                                                                                                                       <  1:103663/139‑1 (MQ=255)
        ttAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTccc                                                                                                              <  1:890463/139‑1 (MQ=255)
                        aTGGACTAATGCGATAAAACTGACATTTTCTTTATTATTATTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGGCCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCAt                                                                                               <  2:835833/139‑1 (MQ=255)
                        aTGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCAt                                                                                               <  2:803359/139‑1 (MQ=255)
                        aTGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCAt                                                                                               <  2:771431/139‑1 (MQ=255)
                          ggACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                             <  2:289903/139‑1 (MQ=255)
                                   cGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTc                                                                                    >  1:818936/1‑139 (MQ=255)
                                     aTAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCtt                                                                                  >  2:133991/1‑139 (MQ=255)
                                      tAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGt                                                                                                    <  1:204785/120‑1 (MQ=255)
                                      tAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGt                                                                                                    >  2:204785/1‑120 (MQ=255)
                                            tGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCAt                                                                                               >  1:525697/1‑119 (MQ=255)
                                            tGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCAt                                                                                               <  2:525697/119‑1 (MQ=255)
                                                      tttATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTa                                                                 <  2:818936/139‑1 (MQ=255)
                                                                  tgctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGtct                                                                                                  <  1:734560/94‑1 (MQ=255)
                                                                  tgctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGtct                                                                                                  >  2:734560/1‑94 (MQ=255)
                                                                   gctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGAc                                                    >  2:463485/1‑139 (MQ=255)
                                                                     tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTa                                                                                        <  2:538762/101‑1 (MQ=255)
                                                                     tgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTa                                                                                        >  1:538762/1‑101 (MQ=255)
                                                                                 tttCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGaa                                      >  2:33998/1‑139 (MQ=255)
                                                                                           gTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACctct                            <  1:133991/139‑1 (MQ=255)
                                                                                           gTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACctct                            <  1:33998/139‑1 (MQ=255)
                                                                                                     cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGaa                                 <  2:674337/124‑1 (MQ=255)
                                                                                                     cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGaa                                 >  1:674337/1‑124 (MQ=255)
                                                                                                          gTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTa                                                                 <  2:405530/87‑1 (MQ=255)
                                                                                                          gTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTa                                                                 >  1:405530/1‑87 (MQ=255)
                                                                                                           tGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGgcg            <  1:435482/139‑1 (MQ=255)
                                                                                                             gTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCGTGATTGTATTTCttt                                                                                 <  2:640321/68‑1 (MQ=255)
                                                                                                             gTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCttt                                                                                 >  1:640321/1‑68 (MQ=255)
                                                                                                                     gACCGCAGAAACGACGATTATGCTTTTTCCCATCATTAGGTCTCACGATTGTATTTCTTTATGATTTTTGTTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACACCAGAATTGGCTCTGACTACGa  >  2:325725/1‑139 (MQ=255)
                                                                                                                     gACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGa  >  2:867157/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                         
ACTTTCTTTTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGACTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGA  >  NC_000913/2104360‑2104615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: