Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,137,277	T→C	intergenic (‑34/‑625)	wza ← / → yegH	colanic acid export protein, outer membrane auxillary lipoprotein/inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,137,277	0	T	C	100.0%	112.7 / NA	32	intergenic (‑34/‑625)	wza/yegH	colanic acid export protein, outer membrane auxillary lipoprotein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/18);  total (14/18)

CTTTGCCCATCGTCGACATATTGCTGCCCGGAAGTACTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCTGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAG  >  NC_000913/2137141‑2137399                                                                                                                                         |                                                                                                                           cTTTGGTCATCGTCGACATATTGCTGCCCGGAAGTAATGAGCAACCGCTTATAAAGGTTACTGACACCAATGATGGCATCAATTTGATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAAAGTAATTATCCcgt                                                                                                                          <  1:216453/139‑1 (MQ=255)    tGCCCATCGTCGACATATTGCTGCCCGGAAGTACTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCa                                                                                                                       <  1:643425/139‑1 (MQ=255)                     ttGCTGCCCGGAAGTACTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCa                                                                                                                       >  2:159841/1‑122 (MQ=255)                     ttGCTGCCCGGAAGTACTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCa                                                                                                                       <  1:159841/122‑1 (MQ=255)                       gctgcCCGGAAGTACTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGc                                                                                                    >  2:342306/1‑139 (MQ=255)                         tgcCCGGAAGTACTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCa                                                                                                  <  1:740446/139‑1 (MQ=255)                         tgcCCGGAAGTACTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCa                                                                                                  <  2:60805/139‑1 (MQ=255)                               gAAGTACTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACt                                                                                                                     >  1:330153/1‑114 (MQ=255)                               gAAGTACTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACt                                                                                                                     <  2:330153/114‑1 (MQ=255)                                     cTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTAcc                                                                                      >  2:172854/1‑139 (MQ=255)                                     cTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTAcc                                                                                      <  2:222513/139‑1 (MQ=255)                                          cAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCcgtg                                                                                                                         <  1:627923/99‑1 (MQ=255)                                          cAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCcgtg                                                                                                                         >  2:627923/1‑99 (MQ=255)                                             ccGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATg                                                                                                <  2:863539/121‑1 (MQ=255)                                             ccGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATg                                                                                                >  1:863539/1‑121 (MQ=255)                                               gCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTCCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTaa                                                                            <  2:109090/139‑1 (MQ=255)                                               gCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTCCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTaa                                                                            >  1:109090/1‑139 (MQ=255)                                                             cTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCtt                                                                                    >  2:875076/1‑117 (MQ=255)                                                             cTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCtt                                                                                    <  1:875076/117‑1 (MQ=255)                                                             cTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCtt                                                                                    >  2:236072/1‑117 (MQ=255)                                                             cTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCtt                                                                                    <  1:236072/117‑1 (MQ=255)                                                                acacCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAAc                                                           >  1:459132/1‑139 (MQ=255)                                                                  acCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACt                                                                                                                     >  1:10976/1‑79 (MQ=255)                                                                  acCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACt                                                                                                                     <  2:10976/79‑1 (MQ=255)                                                                      ataatGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCAt                                                     <  1:734372/139‑1 (MQ=255)                                                                      ataatGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCAt                                                     >  1:681531/1‑139 (MQ=255)                                                                           ggCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCa                                                                                                                       <  2:376619/68‑1 (MQ=255)                                                                           ggCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCa                                                                                                                       >  1:376619/1‑68 (MQ=255)                                                                                                  catcatTGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGc                         <  1:536676/139‑1 (MQ=255)                                                                                                       ttGTTTATTTATCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCt                    <  2:681531/139‑1 (MQ=255)                                                                                                            tatttatCACTTTGGCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGa               >  2:879708/1‑139 (MQ=255)                                                                                                                         ggCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCt                    >  1:766123/1‑121 (MQ=255)                                                                                                                         ggCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCt                    <  2:766123/121‑1 (MQ=255)                                                                                                                         ggCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAg  <  1:879708/139‑1 (MQ=255)                                                                                                                         ggCAGAGTAATTATCCCGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAg  <  1:501858/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                           CTTTGCCCATCGTCGACATATTGCTGCCCGGAAGTACTGTGCAACCGCTTATCAAGGTTACTGACACCAATAATGGCATCAATTTCATTTTGGATTTCATCATTGTTTATTTATCACTTTGGCAGAGTAATTATCCTGTGCACTATTAATAGCAATGTCGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAG  >  NC_000913/2137141‑2137399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑