Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 197,574 T→C S343S (AGT→AGC)  rseP → inner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913197,5740TC100.0% 106.3 / NA 30S343S (AGT→AGC) rsePinner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/15);  total (15/15)

GATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGTATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCG  >  NC_000913/197439‑197697
                                                                                                                                       |                                                                                                                           
gATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATg                                                                                                                          <  1:173562/139‑1 (MQ=255)
  ttGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCt                                                                                                                        >  2:459323/1‑139 (MQ=255)
  ttGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCt                                                                                                                        >  1:824259/1‑139 (MQ=255)
         tGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGaaaa                                                                                                                 >  2:102378/1‑139 (MQ=255)
                    aGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                      >  1:459234/1‑139 (MQ=255)
                    aGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCgg                                                                                                      <  1:102378/139‑1 (MQ=255)
                         aaaGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                 >  1:262896/1‑139 (MQ=255)
                         aaaGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                 >  2:644558/1‑139 (MQ=255)
                               aTTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAAc                                                                                           >  1:441108/1‑139 (MQ=255)
                                      tGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaa                                                                                    <  2:459234/139‑1 (MQ=255)
                                       gCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                   <  2:768148/139‑1 (MQ=255)
                                       gCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                   <  2:823556/139‑1 (MQ=255)
                                                gTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTggg                                                                          <  1:644558/139‑1 (MQ=255)
                                                             cGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaa                                                                                       <  2:66521/113‑1 (MQ=255)
                                                             cGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaa                                                                                       >  1:66521/1‑113 (MQ=255)
                                                                 aGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCaa                                                         <  1:209255/139‑1 (MQ=255)
                                                                            ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                              >  1:213270/1‑139 (MQ=255)
                                                                                      tcgtcgAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGa                                    >  1:301913/1‑139 (MQ=255)
                                                                                              gCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                            <  2:301913/139‑1 (MQ=255)
                                                                                               ccACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                           <  1:63824/139‑1 (MQ=255)
                                                                                               ccACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                           <  1:459323/139‑1 (MQ=255)
                                                                                               ccACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                           <  2:824259/139‑1 (MQ=255)
                                                                                                 aCGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgttg                         >  2:430455/1‑139 (MQ=255)
                                                                                                             tGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaa                                                                                       >  1:292362/1‑65 (MQ=255)
                                                                                                             tGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaa                                                                                       <  2:292362/65‑1 (MQ=255)
                                                                                                               gCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCg           >  2:147138/1‑139 (MQ=255)
                                                                                                                   cTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTc       >  2:272490/1‑139 (MQ=255)
                                                                                                                        gaAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATCTGAAACTGAACAACCTCAGTGGGCCGATCTCGATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTgcg  <  2:605068/139‑1 (MQ=255)
                                                                                                                                cGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                            <  2:323785/105‑1 (MQ=255)
                                                                                                                                cGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                            >  1:323785/1‑105 (MQ=255)
                                                                                                                                       |                                                                                                                           
GATTGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGTATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCG  >  NC_000913/197439‑197697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑