Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,218,207 C→T A117A (GCG→GCA yehY ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,218,2070CT100.0% 114.9 / NA 36A117A (GCG→GCAyehYputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/19);  total (17/19)

GTGAGAGATCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGCGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGTGCACA  >  NC_000913/2218074‑2218341
                                                                                                                                     |                                                                                                                                      
gTGAGAGATCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGtgccgc                                                                                                                                   >  2:673883/1‑139 (MQ=255)
  gagagaTCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcag                                                                                                                                 >  2:488550/1‑139 (MQ=255)
    gagaTCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                               <  1:371903/139‑1 (MQ=255)
    gagaTCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagcc                                                                                                                               <  2:779043/139‑1 (MQ=255)
          gTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGccagccag                                                                                                                         >  1:339179/1‑139 (MQ=255)
                  tGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAAc                                                                                                                     <  2:525568/135‑1 (MQ=255)
                  tGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAAc                                                                                                                     >  1:525568/1‑135 (MQ=255)
                  tGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACt                                                                                                                 >  2:43723/1‑139 (MQ=255)
                   gCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagc                                                                                                                                <  1:805107/123‑1 (MQ=255)
                   gCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGcagc                                                                                                                                >  2:805107/1‑123 (MQ=255)
                                              aatGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTg                                                                                     <  1:235882/139‑1 (MQ=255)
                                                tGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGTCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGcc                                                                                   >  2:876629/1‑139 (MQ=255)
                                                                        aGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGACATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGcc                                                                                                              >  2:34397/1‑88 (MQ=255)
                                                                        aGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGACATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGcc                                                                                                              <  1:34397/88‑1 (MQ=255)
                                                                          cgcCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCt                                                         >  1:445051/1‑139 (MQ=255)
                                                                                aGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAg                                                   <  1:488550/139‑1 (MQ=255)
                                                                                aGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAg                                                   <  1:673883/139‑1 (MQ=255)
                                                                                          tGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGTCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCacac                                         <  1:876629/139‑1 (MQ=255)
                                                                                           ggAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACAcc                                        <  2:793257/139‑1 (MQ=255)
                                                                                            gagaTTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCa                                       <  2:339179/139‑1 (MQ=255)
                                                                                                  cggcggATGGCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATa                                 <  2:445051/139‑1 (MQ=255)
                                                                                                          ggCATCGCTACAGGCCAGCAATGCCAGTGCCGCAGTCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACg                         >  1:7081/1‑139 (MQ=255)
                                                                                                            cATCGCTACAGGCCAGCAATGCCAGTGCCGCAGTCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGaa                       <  2:7081/139‑1 (MQ=255)
                                                                                                                 cTACAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTgcg                  <  2:674030/139‑1 (MQ=255)
                                                                                                                   aCAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTccc                                              >  2:572995/1‑109 (MQ=255)
                                                                                                                   aCAGGCCAGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTccc                                              <  1:572995/109‑1 (MQ=255)
                                                                                                                      ggCCAGCAATGCCAGTGCCGCAGCCAGCCAGAATCCTCTGCCGAGGCTGGTACGCGCCAGAGCACTGCCATTTTGCGCCAGTTGGGTCGCCGACTTTCCAGCTCCCCACACCAGTAATACGAAGACGAATTGCGCCAGa             <  2:516382/139‑1 (MQ=255)
                                                                                                                          aGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCAtt                                                                                >  2:259541/1‑68 (MQ=255)
                                                                                                                          aGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCAtt                                                                                <  1:259541/68‑1 (MQ=255)
                                                                                                                          aGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGa         >  2:684871/1‑139 (MQ=255)
                                                                                                                          aGCAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTGTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGa         <  1:684871/139‑1 (MQ=255)
                                                                                                                          aGCAATGCCAGTGCCGCAGCCAGACAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGa         >  2:354627/1‑139 (MQ=255)
                                                                                                                            cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGt       >  2:364180/1‑139 (MQ=255)
                                                                                                                            cAATGCCAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGt       >  1:759221/1‑139 (MQ=255)
                                                                                                                                 ccAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAg                                                   >  2:250739/1‑90 (MQ=255)
                                                                                                                                 ccAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAg                                                   <  1:250739/90‑1 (MQ=255)
                                                                                                                                 ccAGTGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGTGcaca  >  1:931771/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GTGAGAGATCGTTAAGCGTGCCGGAGTACAGCAACCACAGCGGAATAATGGCAATCTGCATATGCAACAACCAGCGCCACAGCGGATGCGTGGAGATTCGGCGGATGGCATCGCTACAGGCCAGCAATGCCAGCGCCGCAGCCAGCCAGAAACCACTGCCGAGGCTGGTACGCGCCAGCGCACTGCCATTTTGCGCCAGTTGGGTCGCCGCCTTTCCAGCTCCCCACACCAGCAATACGAAGACGAATTGCGCCAGAATGAGTGCACA  >  NC_000913/2218074‑2218341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: