Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,358,163	G→A	A228V (GCG→GTG)	rhmA ←	2‑keto‑3‑deoxy‑L‑rhamnonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,358,163	0	G	A	100.0%	108.7 / NA	35	A228V (GCG→GTG)	rhmA	2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/17);  total (18/17)

TGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGCCC  >  NC_000913/2358029‑2358294                                                                                                                                       |                                                                                                                                    tGGGCCTTAGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCACCCCACACCAg                                                                                                                                 >  1:603653/1‑139 (MQ=255)     ccTTTGATATCAATAACTACCTTTTATGCGTGGCCCA‑TTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCAtt                                                                                                                            >  1:482438/1‑139 (MQ=255)        ttGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgc                                                                                                                          >  1:712233/1‑139 (MQ=255)            tatCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTgcg                                                                                                                      <  2:712233/139‑1 (MQ=255)               cAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCa                                                                                                                   <  1:493107/139‑1 (MQ=255)                        ccTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                       >  2:726354/1‑126 (MQ=255)                        ccTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                       <  1:726354/126‑1 (MQ=255)                            ttATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGcc                                                                                                        <  1:131069/137‑1 (MQ=255)                            ttATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGcc                                                                                                        >  2:131069/1‑137 (MQ=255)                                  gTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAg                                                                                                >  1:359174/1‑139 (MQ=255)                                                ccTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCtt                                                                                  <  2:359174/139‑1 (MQ=255)                                                      ttAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCg                                                                            <  2:119838/139‑1 (MQ=255)                                                              cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                       >  1:584326/1‑88 (MQ=255)                                                              cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                       <  2:584326/88‑1 (MQ=255)                                                                   gTCGTTGATCTGGGGCATCGCTGAACAGCATCGTGGGAATAGCAACCGCGGCCAAGTGTGCATCCCACACCAGGCATGCGTGCGCCATATCAGGGGCTACAACCAGCAAAACAGACGCATTACCCGCAGCACGGATccg                                                               <  2:692642/139‑1 (MQ=255)                                                                               gggCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGt                                                   >  1:779346/1‑139 (MQ=255)                                                                                     cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGa                                                                                               <  1:817043/89‑1 (MQ=255)                                                                                     cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGa                                                                                               >  2:817043/1‑89 (MQ=255)                                                                                     cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCaat                                             >  2:633822/1‑139 (MQ=255)                                                                                          tAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTc                                        <  2:482438/139‑1 (MQ=255)                                                                                                 tCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAg                                                                                                             <  1:931743/63‑1 (MQ=255)                                                                                                 tCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAg                                                                                                             >  2:931743/1‑63 (MQ=255)                                                                                                  cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgc                                                                                                                          <  2:475967/49‑1 (MQ=255)                                                                                                  cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgc                                                                                                                          >  1:475967/1‑49 (MQ=255)                                                                                                  cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCg                                                                            <  1:769658/95‑1 (MQ=255)                                                                                                  cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCg                                                                            >  2:769658/1‑95 (MQ=255)                                                                                                  cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAAtt                                         >  1:704229/1‑130 (MQ=255)                                                                                                  cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAAtt                                         <  2:704229/130‑1 (MQ=255)                                                                                                  cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCa                                  <  1:517059/137‑1 (MQ=255)                                                                                                  cGTGTCAACGCCAACAGCCACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCa                                  >  2:517059/1‑137 (MQ=255)                                                                                                                cAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAg                                                                                                             >  2:262579/1‑48 (MQ=255)                                                                                                                cAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAg                                                                                                             <  1:262579/48‑1 (MQ=255)                                                                                                                   cGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGtt               >  1:506041/1‑139 (MQ=255)                                                                                                                          gTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCggg        <  2:779346/139‑1 (MQ=255)                                                                                                                                tCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGccc  >  1:226416/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    TGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGCCC  >  NC_000913/2358029‑2358294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑