| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,401,654 | G→A | C133C (TGC→TGT) | nuoE ← | NADH:ubiquinone oxidoreductase, chain E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,401,654 | 0 | G | A | 98.2% | 166.9 / NA | 54 | C133C (TGC→TGT) | nuoE | NADH:ubiquinone oxidoreductase, chain E |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (28/25); minor base C (1/0); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGA > NC_000913/2401518‑2401787 | aGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGaca > 1:54097/1‑139 (MQ=255) cGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGacagc > 2:125104/1‑139 (MQ=255) gATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCaa > 1:841794/1‑139 (MQ=255) gATAATGTTTTTCATTTATACCACTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTGGGCAGCAGCGTaagcg < 2:54097/139‑4 (MQ=255) aTAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCg > 2:294242/1‑139 (MQ=255) tAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCgg < 2:216692/139‑1 (MQ=255) aaTGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGCCAGCAAGTTGgcag > 1:100693/1‑125 (MQ=255) aaTGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGgcag < 2:100693/125‑1 (MQ=255) ttCATTTATACCGCTCCCGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGCGTGTCCTCATCGATCATCATGTTTGGCCCTTTACCACAGTTCACCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCaaa > 2:125778/1‑139 (MQ=255) tataCCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGt > 1:842775/1‑139 (MQ=255) cGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTACCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGcc < 1:612322/139‑1 (MQ=255) cTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCt > 2:680124/1‑139 (MQ=255) cTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCt > 2:741472/1‑139 (MQ=255) cTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCt > 2:710626/1‑139 (MQ=255) cTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCt > 1:351104/1‑139 (MQ=255) tCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgcgc > 2:284676/1‑139 (MQ=255) aTGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCgccgcc > 2:426904/1‑139 (MQ=255) gcgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGaa < 1:294242/139‑1 (MQ=255) cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgc > 1:755911/1‑128 (MQ=255) cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgc < 2:755911/128‑1 (MQ=255) cgTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAAt > 2:384126/1‑139 (MQ=255) tCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGAt > 2:503733/1‑139 (MQ=255) tCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGAt < 1:384126/139‑1 (MQ=255) cTCATCGATCATCATGTTTGGCCGTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATaa < 2:841794/139‑1 (MQ=255) cATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc < 2:200988/139‑1 (MQ=255) cATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc < 1:125104/139‑1 (MQ=255) cATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc < 1:284676/139‑1 (MQ=255) cATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc < 1:503733/139‑1 (MQ=255) aTCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgc > 1:344108/1‑115 (MQ=255) aTCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAgc < 2:344108/115‑1 (MQ=255) tCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGt < 2:351104/139‑1 (MQ=255) tCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTACAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGt < 1:680124/139‑1 (MQ=255) gATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTg < 1:426904/139‑1 (MQ=255) atcatGTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGatat > 2:865489/1‑139 (MQ=255) gTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCt < 2:741934/110‑1 (MQ=255) gTTTGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCt > 1:741934/1‑110 (MQ=255) tGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAAt < 2:275619/111‑1 (MQ=255) tGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAAt > 1:275619/1‑111 (MQ=255) tGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc < 1:853092/123‑1 (MQ=255) tGGCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAAcc > 2:853092/1‑123 (MQ=255) gCCCTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCAc > 1:372475/1‑139 (MQ=255) cccTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTg < 1:756806/124‑1 (MQ=255) cccTTTATCACAGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTg > 2:756806/1‑124 (MQ=255) aGTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGTCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATa < 1:311952/139‑1 (MQ=255) gTTCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATaa > 2:70835/1‑139 (MQ=255) ttCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGAt < 2:386346/103‑1 (MQ=255) ttCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGAt > 1:386346/1‑103 (MQ=255) ttCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAAt < 1:885061/136‑1 (MQ=255) ttCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAAt > 2:885061/1‑136 (MQ=255) ttCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAAc > 1:672201/1‑139 (MQ=255) ttCCCCAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAAc > 2:349489/1‑139 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAg < 2:800102/81‑1 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAg > 1:800102/1‑81 (MQ=255) ccccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACgg > 2:63968/1‑139 (MQ=255) cccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGa > 2:717697/1‑139 (MQ=255) cccAGACAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGa < 2:842775/139‑1 (MQ=255) | AGCGGATGCGTTTCGGGAGTACGGATAATGTTTTTCATTTATACCGCTCCAGCAGTTCAGGGATCGCTTCCGGGGTCAGATGCGCGTGAGTGTCCTCATCGATCATCATGTTTGGCCCTTTATCACAGTTCCCCAGGCAGCAAGTTGGCAGCAGCGTAAAGCGGCCATCAAATGTCGTTTGCCCTGGTTTGATGTTCAGCTTTTTCTCGAGCGCCGCCTGAATACCCTGATAACCGTTGATATGACAGACCACGCTGTCACAATAACGGA > NC_000913/2401518‑2401787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |