Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,511,468	C→T	*413* (TAG→TAA)	mntH ←	manganese/divalent cation transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,511,468	0	C	T	100.0%	144.5 / NA	45	*413* (TAG→TAA)	mntH	manganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/23);  total (22/23)

TCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCT  >  NC_000913/2511333‑2511587                                                                                                                                        |                                                                                                                        tCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTAc                                                                                                                      >  1:654541/1‑139 (MQ=255)        aTGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTAc                                                                                                                      <  2:306581/132‑1 (MQ=255)        aTGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTAc                                                                                                                      >  1:306581/1‑132 (MQ=255)              cacaATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCg                                                                                                         >  2:211439/1‑139 (MQ=255)                   tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTcccc                                                                                                    >  1:782686/1‑139 (MQ=255)                      cTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCccacc                                                                                                 <  2:700534/139‑1 (MQ=255)                         aTGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGTGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaac                                                                                              >  2:229569/1‑139 (MQ=255)                          tGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                             <  1:211439/139‑1 (MQ=255)                             gACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAAcc                                                                                          <  2:654541/139‑1 (MQ=255)                               cGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTAc                                                                                                                      >  2:606448/1‑109 (MQ=255)                               cGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTAc                                                                                                                      <  1:606448/109‑1 (MQ=255)                                            tATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCGGCGCCa                                                                           >  2:316821/1‑139 (MQ=255)                                               cAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGa                                                                        <  2:782686/139‑1 (MQ=255)                                                 ggCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCGGCGCCACGAcc                                                                      <  1:316821/139‑1 (MQ=255)                                                   ccTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAg                                                                    >  2:475685/1‑139 (MQ=255)                                                    cTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGAc                                                                       >  2:282886/1‑135 (MQ=255)                                                    cTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGAc                                                                       <  1:282886/135‑1 (MQ=255)                                                     tACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGca                                                                  >  2:142410/1‑139 (MQ=255)                                                      aCAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                                                 <  2:400001/139‑1 (MQ=255)                                                        aaaCCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTcc                                                                                                      <  1:389574/100‑1 (MQ=255)                                                        aaaCCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTcc                                                                                                      >  2:389574/1‑100 (MQ=255)                                                                   ttAACGCGTAGGGCGGATAAGGTGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAg                                                    <  1:229569/139‑1 (MQ=255)                                                                         cgTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtc                                              <  1:475685/139‑1 (MQ=255)                                                                            aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTccc                                                                                                     <  1:336695/81‑1 (MQ=255)                                                                            aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTccc                                                                                                     >  2:336695/1‑81 (MQ=255)                                                                                  gATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                             >  1:555326/1‑83 (MQ=255)                                                                                  gATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                             <  2:555326/83‑1 (MQ=255)                                                                                  gATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTAcg                                     >  1:13391/1‑139 (MQ=255)                                                                                      aGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGtt                                 <  2:629315/139‑1 (MQ=255)                                                                                      aGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGtt                                 <  1:634550/139‑1 (MQ=255)                                                                                         cGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTccc                                                                                                     >  1:117425/1‑68 (MQ=255)                                                                                         cGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTccc                                                                                                     <  2:117425/68‑1 (MQ=255)                                                                                          gTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                             <  2:13391/139‑1 (MQ=255)                                                                                             tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGtt                          >  1:830141/1‑139 (MQ=255)                                                                                             tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGtt                          >  1:78417/1‑139 (MQ=255)                                                                                                                 tGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACt      >  1:71968/1‑139 (MQ=255)                                                                                                                     aGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCt  <  2:78417/139‑1 (MQ=255)                                                                                                                       aTGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                             >  2:633873/1‑110 (MQ=255)                                                                                                                       aTGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                             <  1:633873/110‑1 (MQ=255)                                                                                                                        tGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGca                                                                  <  2:367890/72‑1 (MQ=255)                                                                                                                        tGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGca                                                                  >  1:367890/1‑72 (MQ=255)                                                                                                                             cGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGcc              <  2:353577/119‑1 (MQ=255)                                                                                                                             cGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGcc              >  1:353577/1‑119 (MQ=255)                                                                                                                               cTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAg                                                    <  2:126864/79‑1 (MQ=255)                                                                                                                               cTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAg                                                    >  1:126864/1‑79 (MQ=255)                                                                                                                                        |                                                                                                                        TCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCT  >  NC_000913/2511333‑2511587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑