Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,521,852	T→C	D209G (GAT→GGT)	xapR ←	transcriptional activator of xapAB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,521,852	0	T	C	100.0%	161.9 / NA	47	D209G (GAT→GGT)	xapR	transcriptional activator of xapAB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/22);  total (25/22)

CGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAG  >  NC_000913/2521724‑2521986                                                                                                                                 |                                                                                                                                       cGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagtcagt                                                                                                                              >  1:461895/1‑139 (MQ=255)         tCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                                  <  2:865170/127‑1 (MQ=255)         tCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                                  >  1:865170/1‑127 (MQ=255)             cTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGggcgg                                                                                                                  <  2:401321/139‑1 (MQ=255)              tATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGggcggc                                                                                                                 >  2:251938/1‑139 (MQ=255)                tCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCa                                                                                                                >  1:372744/1‑138 (MQ=255)                tCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCa                                                                                                                <  2:372744/138‑1 (MQ=255)                tCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCAt                                                                                                               <  2:461895/139‑1 (MQ=255)                tCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCAt                                                                                                               <  2:174841/139‑1 (MQ=255)                              aTGTCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGGATTGTAACAAAATACt                                                                                                 >  1:632574/1‑139 (MQ=255)                              aTGCCCATACTGACCATAGCGAGTACCGTTTGCGGATCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATAcc                                                                                                 >  2:567286/1‑138 (MQ=255)                                          aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                                  >  2:85734/1‑94 (MQ=255)                                          aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCcagt                                                                                                                                  <  1:85734/94‑1 (MQ=255)                                          aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAgcg                                                                                     >  2:90941/1‑139 (MQ=255)                                          aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAgcg                                                                                     >  2:889444/1‑139 (MQ=255)                                          aCCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAgcg                                                                                     >  1:802807/1‑139 (MQ=255)                                           ccATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCTCTGCAAAAAACCCCAGTCAGTGTAAACCGGCGGCATTGTAACAAAATACTCGTCACGTAgcgc                                                                                    >  1:560668/1‑139 (MQ=255)                                               aGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTcc                                                                                >  1:403785/1‑139 (MQ=255)                                                    gTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACaa                                                                                                       >  2:209769/1‑111 (MQ=255)                                                    gTACCGTTTGCGGTTCATTAACGTCGCGAATAACAACCGGTGAAAATCCCGCCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACaa                                                                                                       <  1:209769/111‑1 (MQ=255)                                                      aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACa                                                                                                        >  2:394652/1‑108 (MQ=255)                                                      aCCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACa                                                                                                        <  1:394652/108‑1 (MQ=255)                                                        cGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCATAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCACTTCCAGCGGGACg                                                                       >  2:421808/1‑139 (MQ=255)                                                          tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGggcgg                                                                                                                  <  1:584593/94‑1 (MQ=255)                                                          tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGggcgg                                                                                                                  >  2:584593/1‑94 (MQ=255)                                                          tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCa                                                                                                                <  2:337619/96‑1 (MQ=255)                                                          tttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCa                                                                                                                >  1:337619/1‑96 (MQ=255)                                                           ttGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTg                                                                    >  1:407808/1‑139 (MQ=255)                                                            tGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTgg                                                                   >  1:382065/1‑139 (MQ=255)                                                             gCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGa                                                                  <  1:90941/139‑1 (MQ=255)                                                               ggTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGaaa                                                                <  2:403785/139‑1 (MQ=255)                                                                  tCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACggg                                                                                                                     >  1:416930/1‑83 (MQ=255)                                                                  tCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACggg                                                                                                                     <  2:416930/83‑1 (MQ=255)                                                                   cATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAatga                                                            >  1:214644/1‑139 (MQ=255)                                                                       aaCTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATgag                                                        <  2:560668/139‑1 (MQ=255)                                                                             gcgAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCAt                                                                                                               <  1:453847/78‑1 (MQ=255)                                                                             gcgAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCAt                                                                                                               >  2:453847/1‑78 (MQ=255)                                                                               gAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGatgatg                                                <  1:251938/139‑1 (MQ=255)                                                                                          ggTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCa                                     <  2:802807/139‑1 (MQ=255)                                                                                           gTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCAt                                    <  2:407808/139‑1 (MQ=255)                                                                                                  tCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGggcgg                                                                                                                  <  2:412126/54‑1 (MQ=255)                                                                                                  tCCCACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGggcgg                                                                                                                  >  1:412126/1‑54 (MQ=255)                                                                                                    ccACCTGCTGGCAAACTCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAg                           <  1:353191/139‑1 (MQ=255)                                                                                                                    tCGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGc           <  1:889444/139‑1 (MQ=255)                                                                                                                     cGCTGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCa          >  2:435416/1‑139 (MQ=255)                                                                                                                        tGCAAAAAACCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAAcc       >  2:369151/1‑139 (MQ=255)                                                                                                                             aaaaCCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAg  <  1:369151/139‑1 (MQ=255)                                                                                                                             aaaaCCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAg  <  2:150740/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       CGTAGCTGTCCGCTATCAATGTGATACCAATGCCCATACTGACCATAGCGAGTACCGTTTGCGGTTCATTAACTTCGCGAATAACAACCGGTGAAAATCCCACCTGCTGGCAAACTCGCTGCAAAAAATCCCAGTCAGTGTAAACGGGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAG  >  NC_000913/2521724‑2521986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑