Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,552,094 G→A I15I (ATC→ATT)  ypeA ← GNAT family putative N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,552,0940GA100.0% 112.2 / NA 36I15I (ATC→ATT) ypeAGNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/16);  total (20/16)

CATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTG  >  NC_000913/2551962‑2552229
                                                                                                                                    |                                                                                                                                       
cATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCCCGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc                                                                                                                                   >  1:11734/1‑139 (MQ=255)
             aCCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCtt                                                                                                                      >  2:898228/1‑139 (MQ=255)
             aCCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCtt                                                                                                                      >  2:418535/1‑139 (MQ=255)
              ccACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTc                                                                                                                     <  1:898228/139‑1 (MQ=255)
                  gTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTgg                                                                                                                 >  2:764961/1‑139 (MQ=255)
                       cGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaaa                                                                                                            >  2:506658/1‑139 (MQ=255)
                                 aGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAc                                                                                                                                    <  2:823323/105‑1 (MQ=255)
                                 aGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAc                                                                                                                                    >  1:823323/1‑105 (MQ=255)
                                     aCCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                                                                      >  2:123548/1‑131 (MQ=255)
                                     aCCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                                                                      <  1:123548/131‑1 (MQ=255)
                                                    aCGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCt                                                                               >  1:40229/1‑139 (MQ=255)
                                                        cATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCttttt                                                                           >  2:814043/1‑139 (MQ=255)
                                                         aTGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTg                                                                          <  1:193776/139‑1 (MQ=255)
                                                         aTGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTg                                                                          <  1:266132/139‑1 (MQ=255)
                                                                      cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGc                                                             <  1:506658/139‑1 (MQ=255)
                                                                        cTCGATGTCCATTTCCGGTTCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTg                                                           >  2:516118/1‑139 (MQ=255)
                                                                           gATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaa                                                                                                             <  2:852050/86‑1 (MQ=255)
                                                                           gATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaa                                                                                                             >  1:852050/1‑86 (MQ=255)
                                                                            aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt                                                                                                     >  1:52829/1‑93 (MQ=255)
                                                                            aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt                                                                                                     <  2:52829/93‑1 (MQ=255)
                                                                            aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTa                                                       >  1:663359/1‑139 (MQ=255)
                                                                            aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTa                                                       >  1:765961/1‑139 (MQ=255)
                                                                             tGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTAt                                                      >  2:3735/1‑139 (MQ=255)
                                                                                ccATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTTGAAATCTTCCTTGCGAAATACGCGTATCCCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGc                                                   <  2:53178/139‑1 (MQ=255)
                                                                                             gTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGaa                                      <  1:516118/139‑1 (MQ=255)
                                                                                              ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt                                                                                                     >  2:298260/1‑75 (MQ=255)
                                                                                              ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt                                                                                                     <  1:298260/75‑1 (MQ=255)
                                                                                              ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTa                                                                                                    >  2:58224/1‑76 (MQ=255)
                                                                                              ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTa                                                                                                    <  1:58224/76‑1 (MQ=255)
                                                                                                   cGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAAt                                                                                                                         <  2:196971/50‑1 (MQ=255)
                                                                                                   cGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAAt                                                                                                                         >  1:196971/1‑50 (MQ=255)
                                                                                                         cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATg                                                                                            <  1:277455/73‑1 (MQ=255)
                                                                                                         cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATg                                                                                            >  2:277455/1‑73 (MQ=255)
                                                                                                                      cGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCgg             >  1:396056/1‑139 (MQ=255)
                                                                                                                        cTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGtt           <  2:396056/139‑1 (MQ=255)
                                                                                                                                 ggTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTg  <  2:663359/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
CATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAGTG  >  NC_000913/2551962‑2552229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑