Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,655,767 T→C T198A (ACT→GCT)  pepB ← aminopeptidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,655,7670TC100.0% 112.4 / NA 33T198A (ACT→GCT) pepBaminopeptidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/15);  total (18/15)

CTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACC  >  NC_000913/2655633‑2655893
                                                                                                                                      |                                                                                                                              
cTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATa                                                                                                                            <  2:579367/139‑1 (MQ=255)
             ttCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCa                                                                                                               >  2:305020/1‑139 (MQ=255)
                  tCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCTATACCTTTACCTACCAGGCAc                                                                                                          >  1:234199/1‑139 (MQ=255)
                  tCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATAc                                                                                                                           >  2:509334/1‑122 (MQ=255)
                  tCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATAc                                                                                                                           <  1:509334/122‑1 (MQ=255)
                                cAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCTATACCTTTACCTACCAGGCACGCGTACACTGgcgc                                                                                            <  2:234199/139‑1 (MQ=255)
                                        aGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTAt                                                                                    <  2:477129/139‑1 (MQ=255)
                                                 gTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTg                                                                           <  1:604789/139‑1 (MQ=255)
                                                         tGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATAcc                                                                                                                          <  1:635518/84‑1 (MQ=255)
                                                         tGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATAcc                                                                                                                          >  2:635518/1‑84 (MQ=255)
                                                           ccgcgccgcCCATGTCCGTCTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCAcgcg                                                                                                       <  2:350975/101‑1 (MQ=255)
                                                           ccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCAcgcg                                                                                                       >  1:350975/1‑101 (MQ=255)
                                                                              cTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGga                                              >  1:355630/1‑139 (MQ=255)
                                                                                  aTCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAAc                                          >  1:682487/1‑139 (MQ=255)
                                                                                   tCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACg                                         >  1:682366/1‑139 (MQ=255)
                                                                                                     tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTg                                                                           <  1:522066/87‑1 (MQ=255)
                                                                                                     tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTg                                                                           >  2:522066/1‑87 (MQ=255)
                                                                                                         attGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtg                   >  2:581389/2‑139 (MQ=255)
                                                                                                              tGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg              <  1:305020/139‑1 (MQ=255)
                                                                                                               gCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc             <  1:115458/139‑1 (MQ=255)
                                                                                                                 tGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                             >  2:919349/1‑41 (MQ=255)
                                                                                                                 tGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAgg                                                                                                             <  1:919349/41‑1 (MQ=255)
                                                                                                                   tAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCa         >  1:521242/1‑139 (MQ=255)
                                                                                                                       cgccgGAGTCAAAAGCGAAACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGtt                                                                       >  2:389385/1‑73 (MQ=255)
                                                                                                                       cgccgGAGTCAAAAGCGAAACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGtt                                                                       <  1:389385/73‑1 (MQ=255)
                                                                                                                         ccgGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAAc   >  2:548538/1‑139 (MQ=255)
                                                                                                                          cgGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAAcc  >  1:647116/1‑139 (MQ=255)
                                                                                                                           ggAGTCAAAAGCGATACCTTTACCTGCCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATc                                                                 <  1:599874/75‑1 (MQ=255)
                                                                                                                           ggAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATc                                                                 >  2:599874/1‑75 (MQ=255)
                                                                                                                             aGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                            <  1:898664/94‑1 (MQ=255)
                                                                                                                             aGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                            >  2:898664/1‑94 (MQ=255)
                                                                                                                                cAAAAGCGATACCTTTACCTACCAGGCACGCGTaca                                                                                                   >  1:887690/1‑36 (MQ=255)
                                                                                                                                cAAAAGCGATACCTTTACCTACCAGGCACGCGTaca                                                                                                   <  2:887690/36‑1 (MQ=255)
                                                                                                                                      |                                                                                                                              
CTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACC  >  NC_000913/2655633‑2655893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: