Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 30,616 T→C P322P (CCT→CCC carA → carbamoyl phosphate synthetase small subunit, glutamine amidotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091330,6160TC100.0% 84.6 / NA 25P322P (CCT→CCCcarAcarbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/12);  total (13/12)

CGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCGC  >  NC_000913/30487‑30743
                                                                                                                                 |                                                                                                                               
cGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTg                                                                                                                        >  1:417086/1‑139 (MQ=255)
                             gTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCa                                                                                                                 <  1:59022/117‑1 (MQ=255)
                             gTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCa                                                                                                                 >  2:59022/1‑117 (MQ=255)
                                        ggcAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAgg                                                                                <  1:912391/139‑1 (MQ=255)
                                                                 agaAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGc                                                       >  1:772968/1‑139 (MQ=255)
                                                                        cGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTAc                                                                                   <  2:836709/104‑1 (MQ=255)
                                                                        cGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTAc                                                                                   >  1:836709/1‑104 (MQ=255)
                                                                        cGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGc                                                >  2:216038/1‑139 (MQ=255)
                                                                                 gATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTa                                                                                         <  2:227303/89‑1 (MQ=255)
                                                                                 gATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTa                                                                                         >  1:227303/1‑89 (MQ=255)
                                                                                       cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCAtt                                                    <  1:140725/120‑1 (MQ=255)
                                                                                       cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCAtt                                                    >  2:140725/1‑120 (MQ=255)
                                                                                       cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCt                                 >  2:882811/1‑139 (MQ=255)
                                                                                         cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCgtgt                                                                                                                   >  2:479096/1‑55 (MQ=255)
                                                                                         cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCgtgt                                                                                                                   <  1:479096/55‑1 (MQ=255)
                                                                                         cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTAc                                                                                   >  1:231337/1‑87 (MQ=255)
                                                                                         cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTAc                                                                                   <  2:231337/87‑1 (MQ=255)
                                                                                            aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagc                            <  1:216038/139‑1 (MQ=255)
                                                                                              aaCCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa                                                                                              >  2:630402/1‑71 (MQ=255)
                                                                                              aaCCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa                                                                                              <  1:630402/71‑1 (MQ=255)
                                                                                                   cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACg                                                                                            <  1:789018/68‑1 (MQ=255)
                                                                                                   cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACg                                                                                            >  2:789018/1‑68 (MQ=255)
                                                                                                   cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGACATTCATCGCa                                                                    <  2:753156/92‑1 (MQ=255)
                                                                                                   cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGACATTCATCGCa                                                                    >  1:753156/1‑92 (MQ=255)
                                                                                                                      gCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGAcgccgcgc  <  1:882811/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                               
CGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCGC  >  NC_000913/30487‑30743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: