Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,966,188 C→T *749* (TAG→TAA ptsP ← PEP‑protein phosphotransferase enzyme I, GAF domain containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,966,1880CT100.0% 142.3 / NA 45*749* (TAG→TAAptsPPEP‑protein phosphotransferase enzyme I, GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/19);  total (26/19)

CTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATGCGC  >  NC_000913/2966053‑2966323
                                                                                                                                       |                                                                                                                                       
cTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTtat                                                                                                                                      <  2:874644/139‑1 (MQ=255)
                          cGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCAt                                                                                                            >  2:690423/1‑139 (MQ=255)
                            aTTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAg                                                                                                                     >  2:429325/1‑128 (MQ=255)
                            aTTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAg                                                                                                                     <  1:429325/128‑1 (MQ=255)
                                    gcgcgcCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGc                                                                                                  <  1:690423/139‑1 (MQ=255)
                                                                      aataatAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCgg                                                                >  1:306172/1‑139 (MQ=255)
                                                                                   ggggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAg                                                                                                                     >  1:137043/1‑73 (MQ=255)
                                                                                   ggggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAg                                                                                                                     <  2:137043/73‑1 (MQ=255)
                                                                                     ggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCtt                                                 <  2:91159/139‑1 (MQ=255)
                                                                                             ctggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATc                                                                                                                       <  2:734039/61‑1 (MQ=255)
                                                                                             ctggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATc                                                                                                                       >  1:734039/1‑61 (MQ=255)
                                                                                              tggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATaa                                                                                           >  2:375985/1‑88 (MQ=255)
                                                                                              tggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATaa                                                                                           <  1:375985/88‑1 (MQ=255)
                                                                                                  tGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACg                                                                                                   <  2:711995/76‑1 (MQ=255)
                                                                                                  tGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACg                                                                                                   >  1:711995/1‑76 (MQ=255)
                                                                                                             gTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGAtttt                         <  1:893400/139‑1 (MQ=255)
                                                                                                              ttAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCcgc                                                                                                       >  2:177399/1‑60 (MQ=255)
                                                                                                              ttAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCcgc                                                                                                       <  1:177399/60‑1 (MQ=255)
                                                                                                                     atatGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCt                    <  1:362277/136‑1 (MQ=255)
                                                                                                                     atatGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCt                    >  2:362277/1‑136 (MQ=255)
                                                                                                                      tatGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCgg                >  1:471343/1‑139 (MQ=255)
                                                                                                                      tatGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCgg                >  1:912467/1‑139 (MQ=255)
                                                                                                                       atGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCa                                >  2:273393/1‑122 (MQ=255)
                                                                                                                       atGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCa                                <  1:273393/122‑1 (MQ=255)
                                                                                                                       atGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGc               <  1:341205/139‑1 (MQ=255)
                                                                                                                       atGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGc               >  2:341205/1‑139 (MQ=255)
                                                                                                                        tGTATATGATCCGCGTTATAACCCTCCGCGAATCAgcccgc                                                                                                                >  2:444937/1‑41 (MQ=255)
                                                                                                                        tGTATATGATCCGCGTTATAACCCTCCGCGAATCAgcccgc                                                                                                                <  1:444937/41‑1 (MQ=255)
                                                                                                                        tGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCcgcg                                                                                                      >  1:844604/1‑51 (MQ=255)
                                                                                                                        tGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCcgcg                                                                                                      <  2:844604/51‑1 (MQ=255)
                                                                                                                             atGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATc         >  1:302813/1‑139 (MQ=255)
                                                                                                                               gATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCaa       >  1:338777/1‑139 (MQ=255)
                                                                                                                               gATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCaa       >  1:597591/1‑139 (MQ=255)
                                                                                                                               gATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCaa       >  1:261536/1‑139 (MQ=255)
                                                                                                                                aTCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCcgcg                                                                                                      >  2:474260/1‑43 (MQ=255)
                                                                                                                                aTCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCcgcg                                                                                                      <  1:474260/43‑1 (MQ=255)
                                                                                                                                aTCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAAt      >  1:354208/1‑139 (MQ=255)
                                                                                                                                 tCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGc                                                                                                  >  2:122254/1‑46 (MQ=255)
                                                                                                                                 tCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGc                                                                                                  <  1:122254/46‑1 (MQ=255)
                                                                                                                                  ccGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACg                                                                                                   <  2:87275/44‑1 (MQ=255)
                                                                                                                                  ccGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACg                                                                                                   >  1:87275/1‑44 (MQ=255)
                                                                                                                                  ccGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATgc    >  1:842777/1‑139 (MQ=255)
                                                                                                                                  ccGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGATCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATgc    >  2:286838/1‑139 (MQ=255)
                                                                                                                                   cgcgTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCGATa                                                                                            <  2:124599/50‑1 (MQ=255)
                                                                                                                                   cgcgTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATa                                                                                            >  1:124599/1‑50 (MQ=255)
                                                                                                                                    gcgtTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTc                                                                                                >  2:439653/1‑45 (MQ=255)
                                                                                                                                    gcgtTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTg                                                      >  1:281048/1‑87 (MQ=255)
                                                                                                                                    gcgtTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTg                                                      <  2:281048/87‑1 (MQ=255)
                                                                                                                                    gcgtTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATgcgc  >  2:304491/1‑139 (MQ=255)
                                                                                                                                    gcgtTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATgcgc  >  2:287300/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATGCGC  >  NC_000913/2966053‑2966323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: