Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,967,997	A→G	L146L (CTT→CTC)	ptsP ←	PEP‑protein phosphotransferase enzyme I, GAF domain containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,967,997	0	A	G	100.0%	160.6 / NA	46	L146L (CTT→CTC)	ptsP	PEP‑protein phosphotransferase enzyme I, GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/23);  total (23/23)

GTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCAAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTT  >  NC_000913/2967861‑2968124                                                                                                                                         |                                                                                                                                gTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAg                                                                                                                               <  1:886801/139‑1 (MQ=255)  tAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGACAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGc                                                                                                                              <  2:819132/139‑1 (MQ=255)   aaCGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCTCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCg                                                                                                                             >  2:898182/1‑139 (MQ=255)     cGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTc                                                                                                                           >  2:529753/1‑139 (MQ=255)     cGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTc                                                                                                                           >  1:674290/1‑139 (MQ=255)         gCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCa                                                                                                                          >  2:64440/1‑136 (MQ=255)         gCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCa                                                                                                                          <  1:64440/136‑1 (MQ=255)                 ccAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCTCGTCTGGCGATATTGTCCAAACAAGGCAGTCAACTGACACTGATAAAGAATAGCCGCCATCTGGGTGACGAAGGTCACCCAGAAGGAt                                                                                                               >  2:701523/1‑139 (MQ=255)                     ccTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGAttctt                                                                                                           <  1:804087/139‑1 (MQ=255)                               tCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGt                                                                                                  >  2:736323/1‑138 (MQ=255)                               tCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGt                                                                                                  <  1:736323/138‑1 (MQ=255)                                                gcCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAActct                                                                                 >  2:838081/1‑138 (MQ=255)                                                gcCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAActct                                                                                 <  1:838081/138‑1 (MQ=255)                                                gcCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACtctc                                                                                >  1:658380/1‑139 (MQ=255)                                                gcCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACtctc                                                                                <  1:898182/139‑1 (MQ=255)                                                    gTAATGCGCGGATTCGCGTCTGGCGATGTTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCACAACTCTCGTTg                                                                            >  2:554564/1‑139 (MQ=255)                                                          cgcgGATTCGCGTCTGGCGATATTGCCCAAACAAGACAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACttt                                                                                                     >  2:755008/1‑108 (MQ=255)                                                          cgcgGATTCGCGTCTGGCGATATTGCCCAAACAAGACAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACttt                                                                                                     <  1:755008/108‑1 (MQ=255)                                                          cgcgGATTCGCGTCTGGCGATATTGCCCAAAAAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTac                                                                      <  1:701523/139‑1 (MQ=255)                                                                                   cccAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGa                                             >  2:197220/1‑139 (MQ=255)                                                                                   cccAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGa                                             >  1:225431/1‑139 (MQ=255)                                                                                      aaaCAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGt                                          <  1:529753/139‑1 (MQ=255)                                                                                       aaCAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAg                                                                                                                               <  1:215027/53‑1 (MQ=255)                                                                                       aaCAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAg                                                                                                                               >  2:215027/1‑53 (MQ=255)                                                                                            ggCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGaata                                    <  2:674290/139‑1 (MQ=255)                                                                                             gCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTa                                                              >  1:432821/1‑112 (MQ=255)                                                                                             gCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTa                                                              <  2:432821/112‑1 (MQ=255)                                                                                                 tCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCAcc                                                                                                                        <  1:749813/50‑1 (MQ=255)                                                                                                 tCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCAcc                                                                                                                        >  2:749813/1‑50 (MQ=255)                                                                                                  cAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTac                                                                      >  2:646252/1‑99 (MQ=255)                                                                                                  cAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTac                                                                      <  1:646252/99‑1 (MQ=255)                                                                                                  cAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGt                              <  2:752957/139‑1 (MQ=255)                                                                                                      tGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGc                          >  1:249599/1‑139 (MQ=255)                                                                                                               gaaagaaTAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATAc                                                                                             <  2:868560/63‑1 (MQ=255)                                                                                                               gaaagaaTAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATAc                                                                                             >  1:868560/1‑63 (MQ=255)                                                                                                                 aagaaTAGCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAAcgcg               <  2:225431/139‑1 (MQ=255)                                                                                                                        gCTGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAAc        >  2:507234/1‑139 (MQ=255)                                                                                                                          tGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACt                                                                                                       <  2:907672/42‑1 (MQ=255)                                                                                                                          tGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACt                                                                                                       >  1:907672/1‑42 (MQ=255)                                                                                                                          tGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGtt                                                                             >  2:423091/1‑68 (MQ=255)                                                                                                                          tGCCATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGtt                                                                             <  1:423091/68‑1 (MQ=255)                                                                                                                            ccATCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGttc    <  1:853471/139‑1 (MQ=255)                                                                                                                              aTCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTac                                                                      >  2:375643/1‑71 (MQ=255)                                                                                                                              aTCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTac                                                                      <  1:375643/71‑1 (MQ=255)                                                                                                                              aTCTGGGTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGttctt  >  1:478132/1‑139 (MQ=255)                                                                                                                                   ggTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGc                                                                           <  2:466153/61‑1 (MQ=255)                                                                                                                                   ggTGGCGAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGc                                                                           >  1:466153/1‑61 (MQ=255)                                                                                                                                         |                                                                                                                                GTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGCTGCCATCTGGGTGGCAAGCGTCACCAGGAAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTT  >  NC_000913/2967861‑2968124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑