Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	309,358	C→T	*223* (TAG→TAA)	ecpB ←	ECP production pilus chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	309,358	0	C	T	100.0%	130.3 / NA	41	*223* (TAG→TAA)	ecpB	ECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/22);  total (19/22)

CGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCG  >  NC_000913/309231‑309493                                                                                                                                |                                                                                                                                        cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACggg                                                                                                                              >  1:469951/1‑139 (MQ=255) cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACg                                                                                                                                <  1:895095/137‑1 (MQ=255) cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACg                                                                                                                                >  2:895095/1‑137 (MQ=255)            cagcagCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTAt                                                                                                                   <  2:469951/139‑1 (MQ=255)                      ggCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCa                                                                                                         <  1:74934/139‑1 (MQ=255)                                                ccATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCg                                                                               <  2:814831/139‑1 (MQ=255)                                                  aTGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCa                                                                             <  2:405559/139‑1 (MQ=255)                                                  aTGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCa                                                                             >  1:918479/1‑139 (MQ=255)                                                       gAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAccc                                                                                              >  1:416797/1‑117 (MQ=255)                                                       gAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAccc                                                                                              <  2:416797/117‑1 (MQ=255)                                                              aaCTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCgg                                                                 >  1:73432/1‑139 (MQ=255)                                                                             ccTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGAc                                                  <  1:768708/139‑1 (MQ=255)                                                                                      aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATg                                                                                                                          >  2:82869/1‑58 (MQ=255)                                                                                      aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATg                                                                                                                          <  1:82869/58‑1 (MQ=255)                                                                                      aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTAt                                                                                  >  2:123921/1‑98 (MQ=255)                                                                                      aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTAt                                                                                  <  1:123921/98‑1 (MQ=255)                                                                                        ccGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCAt                                       >  1:68880/1‑139 (MQ=255)                                                                                           tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCttt                                                                                       <  1:579769/88‑1 (MQ=255)                                                                                           tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCttt                                                                                       >  2:579769/1‑88 (MQ=255)                                                                                           tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCt                                                                                         <  1:527661/86‑1 (MQ=255)                                                                                           tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCt                                                                                         >  2:527661/1‑86 (MQ=255)                                                                                                      ttGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATtct                         <  2:38999/139‑1 (MQ=255)                                                                                                       tGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATtctc                        >  2:226459/1‑139 (MQ=255)                                                                                                         tCGTTATCCATCTGCACAGCTATTTATTTCACGGGa                                                                                                                             >  1:467644/1‑36 (MQ=255)                                                                                                         tCGTTATCCATCTGCACAGCTATTTATTTCACGGGa                                                                                                                             <  2:467644/36‑1 (MQ=255)                                                                                                           gTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCACAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTa                    <  1:755108/139‑1 (MQ=255)                                                                                                           gTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTa                    <  1:687078/139‑1 (MQ=255)                                                                                                            ttATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTac                   <  1:226459/139‑1 (MQ=255)                                                                                                            ttATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTac                   <  2:745259/139‑1 (MQ=255)                                                                                                             tATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGc                                                                              <  1:723408/79‑1 (MQ=255)                                                                                                             tATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGc                                                                              >  2:723408/1‑79 (MQ=255)                                                                                                                   tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCTAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTac                   <  2:620072/132‑1 (MQ=255)                                                                                                                   tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCa                                                                             <  1:187879/74‑1 (MQ=255)                                                                                                                   tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCa                                                                             >  2:187879/1‑74 (MQ=255)                                                                                                                   tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAAtt                           <  1:481784/124‑1 (MQ=255)                                                                                                                   tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAAtt                           >  2:481784/1‑124 (MQ=255)                                                                                                                   tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTac                   >  1:620072/1‑132 (MQ=255)                                                                                                                   tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCtt            >  1:497451/1‑139 (MQ=255)                                                                                                                       cacaGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCg        >  1:164564/1‑139 (MQ=255)                                                                                                                         caGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGtt      >  1:453291/1‑139 (MQ=255)                                                                                                                           gCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTgg    <  2:164564/139‑1 (MQ=255)                                                                                                                            ctatttatTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAAtt                           <  2:455219/115‑1 (MQ=255)                                                                                                                            ctatttatTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAAtt                           >  1:455219/1‑115 (MQ=255)                                                                                                                             tatttattTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTggcg  <  2:73432/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                        CGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCG  >  NC_000913/309231‑309493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑