Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,187,415	G→A	*302* (TAG→TAA)	insD1 →	IS2 transposase TnpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,187,415	0	G	A	100.0%	72.6 / NA	25	*302* (TAG→TAA)	insD1	IS2 transposase TnpB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/9);  total (16/9)

GGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGG  >  NC_000913/3187318‑3187548                                                                                                  |                                                                                                                                      ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCg                                                                                                       <  2:292267/130‑1 (MQ=255) ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCg                                                                                                       >  1:292267/1‑130 (MQ=255) ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTAt                                                                                              >  1:358316/1‑139 (MQ=255)              tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGt                                                                                                      >  2:399578/1‑118 (MQ=255)              tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGt                                                                                                      <  1:399578/118‑1 (MQ=255)                                                  gCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGt                                                                                                                          <  2:64518/62‑1 (MQ=14)                                                  gCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGt                                                                                                                          >  1:64518/1‑62 (MQ=9)                                                      cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCg                                                                  <  1:283903/114‑1 (MQ=255)                                                      cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCg                                                                  >  2:283903/1‑114 (MQ=255)                                                      cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTaa                                         >  1:413065/1‑139 (MQ=255)                                                                aTGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGa                                                  <  2:927723/120‑1 (MQ=255)                                                                aTGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGa                                                  >  1:927723/1‑120 (MQ=255)                                                                   ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATg                                                                                             <  1:469346/74‑1 (MQ=255)                                                                   ggTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATg                                                                                             >  2:469346/1‑74 (MQ=255)                                                                        aGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAAc                       >  1:529650/1‑139 (MQ=255)                                                                             tAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCg                                                                  <  2:267912/91‑1 (MQ=255)                                                                             tAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCg                                                                  >  1:267912/1‑91 (MQ=255)                                                                             tAACAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATg                  >  1:436404/1‑139 (MQ=255)                                                                              aaCAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATgg                 >  2:264338/1‑139 (MQ=255)                                                                              aaCAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATgg                 >  1:171644/1‑139 (MQ=255)                                                                              aaCAGATGTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATgg                 >  1:140373/1‑139 (MQ=255)                                                                                     gTCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTa          >  1:89017/1‑139 (MQ=255)                                                                                      tCTGGAAATATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTAc         >  2:224816/1‑139 (MQ=255)                                                                                           aaaTATAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTc    <  2:426258/139‑1 (MQ=255)                                                                                             atatAAGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCgg  <  1:224816/139‑1 (MQ=255)                                                                                                  |                                                                                                                                      GGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGG  >  NC_000913/3187318‑3187548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑