Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,218,365	C→T	E238K (GAG→AAG)	aer ←	fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,218,365	0	C	T	100.0%	98.7 / NA	32	E238K (GAG→AAG)	aer	fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)

TTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACA  >  NC_000913/3218232‑3218500                                                                                                                                      |                                                                                                                                        ttcagttcaTCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGc                                                                                                                                    >  2:499053/1‑139 (MQ=255)     gttcaTCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCg                                                                                                                                >  1:700538/1‑139 (MQ=255)           cGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAg                                                                                                                          <  2:607730/139‑1 (MQ=255)                ccTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                                     <  2:610516/139‑1 (MQ=255)                    tCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAac                                                                                                                 >  1:519342/1‑139 (MQ=255)                     cGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAaca                                                                                                                <  1:287415/139‑1 (MQ=255)                                     ccATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCgg                                                                                                >  2:119245/1‑139 (MQ=255)                                        tttCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                                     >  2:647468/1‑115 (MQ=255)                                        tttCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                                     <  1:647468/115‑1 (MQ=255)                                           cTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCa                                                                                          <  1:119245/139‑1 (MQ=255)                                                    gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCa                                                                                          >  1:65466/1‑130 (MQ=255)                                                    gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCa                                                                                          <  2:65466/130‑1 (MQ=255)                                                    gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTg                                                                                 >  2:724781/1‑139 (MQ=255)                                                               tGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                                      <  2:700538/139‑1 (MQ=255)                                                               tGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                                      <  2:519342/139‑1 (MQ=255)                                                                     aTCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTg                                                                             <  1:357321/126‑1 (MQ=255)                                                                     aTCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTg                                                                             >  2:357321/1‑126 (MQ=255)                                                                                 ccAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                                     >  2:97050/1‑74 (MQ=255)                                                                                 ccAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                                     <  1:97050/74‑1 (MQ=255)                                                                                   aaCGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAaca                                                                                                                <  2:689152/77‑1 (MQ=255)                                                                                   aaCGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAaca                                                                                                                >  1:689152/1‑77 (MQ=255)                                                                                      ggCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGa                                                                                                                               <  2:117223/59‑1 (MQ=255)                                                                                      ggCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGa                                                                                                                               >  1:117223/1‑59 (MQ=255)                                                                                         acaTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTg                                            <  1:499053/139‑1 (MQ=255)                                                                                                   aaGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCaaaa                                  >  1:673161/1‑139 (MQ=255)                                                                                                          ccTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGgcgc                           >  2:198968/1‑139 (MQ=255)                                                                                                            tACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCtt                         <  2:673161/139‑1 (MQ=255)                                                                                                                gCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCca                     >  1:139215/1‑139 (MQ=255)                                                                                                                      aaTGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATa               >  2:253050/1‑139 (MQ=255)                                                                                                                                ccAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                                      >  1:554911/1‑74 (MQ=255)                                                                                                                                ccAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                                      <  2:554911/74‑1 (MQ=255)                                                                                                                                   gCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGcaca  <  1:787434/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACA  >  NC_000913/3218232‑3218500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑