Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,219,088	G→A	intergenic (‑12/‑406)	aer ← / → patA	fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component/putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,219,088	0	G	A	100.0%	146.5 / NA	47	intergenic (‑12/‑406)	aer/patA	fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component/putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)

GCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTT  >  NC_000913/3218954‑3219223                                                                                                                                       |                                                                                                                                        gctcgccgacccaAAAGTGTCATTAGGATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCa                                                                                                                                     <  2:565121/128‑1 (MQ=255) gCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCa                                                                                                                                     <  2:25078/139‑1 (MQ=255) gCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCa                                                                                                                                     <  2:630745/139‑1 (MQ=255)   tCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAgg                                                                                                                                   >  1:279215/1‑139 (MQ=255)    cACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGt                                                                                                                                  >  1:345853/1‑139 (MQ=255)                           cATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTc                                                                                                           <  1:670949/139‑1 (MQ=255)                                     ataGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTc                                                                                                           <  1:684357/129‑1 (MQ=255)                                     ataGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTc                                                                                                           >  2:684357/1‑129 (MQ=255)                                         cTTTTCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACAGATGAGAAGACATGTTCGCGTCCTATGc                                                                                                                                      >  2:541570/1‑98 (MQ=255)                                         cTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGc                                                                                                                                      <  1:541570/98‑1 (MQ=255)                                         cTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCgg                                                                                             >  2:347099/1‑139 (MQ=255)                                              cAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCg                                                                                        >  1:712588/1‑139 (MQ=255)                                              cAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCg                                                                                        >  1:907469/1‑139 (MQ=255)                                                    ggTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCgg                                                                                  <  1:137784/139‑1 (MQ=255)                                                                cAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTtata                                                                                                                              >  1:6541/1‑83 (MQ=255)                                                                cAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTtata                                                                                                                              <  2:6541/83‑1 (MQ=255)                                                                cAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAActt                                                                                                                 >  2:838399/1‑96 (MQ=255)                                                                cAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAActt                                                                                                                 <  1:838399/96‑1 (MQ=255)                                                                cAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTg                                                                                     <  1:394528/124‑1 (MQ=255)                                                                cAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTg                                                                                     >  2:394528/1‑124 (MQ=255)                                                                cAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAAcc                                                                      >  2:913855/1‑139 (MQ=255)                                                                  gagTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGa                                                                    <  2:907469/139‑1 (MQ=255)                                                                     tGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTatat                                                                                                                             >  2:440090/1‑79 (MQ=255)                                                                     tGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTatat                                                                                                                             <  1:440090/79‑1 (MQ=255)                                                                       gTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATCGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAAt                                                               <  2:600396/139‑1 (MQ=255)                                                                        tATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCa                                                                                                                                     >  2:156530/1‑68 (MQ=255)                                                                        tATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCa                                                                                                                                     <  1:156530/68‑1 (MQ=255)                                                                                 ccAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGc                                                                                                                                      <  2:208302/58‑1 (MQ=255)                                                                                 ccAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGc                                                                                                                                      >  1:208302/1‑58 (MQ=255)                                                                                        ggTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTGGATTTAGATCGCAAt                                              >  2:738548/1‑139 (MQ=255)                                                                                                      tGACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAaca                                >  2:839030/1‑139 (MQ=255)                                                                                                       gACATACGGATGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAacac                               <  2:712588/139‑1 (MQ=255)                                                                                                                aTGAGAAGACATGTTCGCTTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTa                                                                         <  1:419065/88‑1 (MQ=255)                                                                                                                aTGAGAAGACATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTa                                                                         >  2:419065/1‑88 (MQ=255)                                                                                                                   agaagaCATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCgg                                                                                  <  1:411235/76‑1 (MQ=255)                                                                                                                   agaagaCATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCgg                                                                                  >  2:411235/1‑76 (MQ=255)                                                                                                                    gaagaCATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTaa                                                                        >  2:110823/1‑85 (MQ=255)                                                                                                                    gaagaCATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTaa                                                                        <  1:110823/85‑1 (MQ=255)                                                                                                                      agaCATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCATAGTTTATGTCGGCCCCGCTGCGGTTATCTTCAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTg                <  2:139215/139‑1 (MQ=255)                                                                                                                       gaCATGTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGa               >  2:832549/1‑139 (MQ=255)                                                                                                                            gTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGt                                                                                                <  2:697041/53‑1 (MQ=255)                                                                                                                            gTTCGCGTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGt                                                                                                >  1:697041/1‑53 (MQ=255)                                                                                                                               cgcgTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTa                                    >  1:202618/1‑110 (MQ=255)                                                                                                                               cgcgTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTa                                    <  2:202618/110‑1 (MQ=255)                                                                                                                               cgcgTCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAAt       >  1:547803/1‑139 (MQ=255)                                                                                                                                   tCCTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACtt   <  2:279215/139‑1 (MQ=255)                                                                                                                                    ccTATGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACttt  <  2:547803/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTT  >  NC_000913/3218954‑3219223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑