Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 323,355 C→T P340S (CCA→TCA)  ykgF → ferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913323,3550CT100.0% 169.8 / NA 54P340S (CCA→TCA) ykgFferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/29);  total (25/29)

TTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTC  >  NC_000913/323231‑323490
                                                                                                                            |                                                                                                                                       
ttCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTg                                                                                                                           >  1:456272/1‑139 (MQ=255)
                                 gctgcGCTGTATTCGCTGCGGGGCTTGCATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCCATTGGTGCGGTGATTACTCCGCTACTTGGCGGCTAtaaagatttt                                                                                          <  2:536476/139‑1 (MQ=255)
                                              cGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCt                                                                             <  1:887736/139‑1 (MQ=255)
                                                  gCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGc                                                                         <  2:414973/139‑1 (MQ=255)
                                                    ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGt                                                                                                                                 >  1:129690/1‑81 (MQ=255)
                                                    ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGt                                                                                                                                 <  2:129690/81‑1 (MQ=255)
                                                    ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                                       <  2:499566/139‑1 (MQ=255)
                                                    ggggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                                       >  2:159129/1‑139 (MQ=255)
                                                      ggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     <  2:628740/139‑1 (MQ=255)
                                                      ggCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     <  1:159129/139‑1 (MQ=255)
                                                                    cTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGc                                                       <  1:53149/139‑1 (MQ=255)
                                                                     ttGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGcaca                                                         <  2:702435/136‑1 (MQ=255)
                                                                     ttGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGcaca                                                         >  1:702435/1‑136 (MQ=255)
                                                                      tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTtctc                                                                                                                    <  1:84869/76‑1 (MQ=255)
                                                                      tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTtctc                                                                                                                    >  2:84869/1‑76 (MQ=255)
                                                                      tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                         >  1:325089/1‑87 (MQ=255)
                                                                      tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTggc                                                                                                         <  2:325089/87‑1 (MQ=255)
                                                                      tGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCtt                                                     >  1:248785/1‑139 (MQ=255)
                                                                          cGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGa                                                 >  1:43167/1‑139 (MQ=255)
                                                                           ggCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGAc                                                <  2:248785/139‑1 (MQ=255)
                                                                            gCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     <  1:496921/117‑1 (MQ=255)
                                                                            gCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     >  2:496921/1‑117 (MQ=255)
                                                                              atatCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAAt                                                                                                                                     >  2:154611/1‑51 (MQ=255)
                                                                              atatCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAAt                                                                                                                                     <  1:154611/51‑1 (MQ=255)
                                                                                  cGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgt                                         <  1:14874/139‑1 (MQ=255)
                                                                                  cGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTAAGCCTGCTCTTTATGCACAGCTTGTGACAACgtgt                                         >  2:171364/1‑139 (MQ=255)
                                                                                      atatTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTcc                                     <  2:43167/139‑1 (MQ=255)
                                                                                      atatTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTcc                                     <  1:171364/139‑1 (MQ=255)
                                                                                       tatTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaag                                                                                               <  1:228016/80‑1 (MQ=255)
                                                                                       tatTGGCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaag                                                                                               >  2:228016/1‑80 (MQ=255)
                                                                                            gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgtgt                                       >  2:378382/1‑131 (MQ=255)
                                                                                            gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgtgt                                       <  1:378382/131‑1 (MQ=255)
                                                                                            gCGGTCATGGATATGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCg                               >  2:273492/1‑139 (MQ=255)
                                                                                                         tGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                           >  2:574044/1‑98 (MQ=255)
                                                                                                         tGGCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGca                                                           <  1:574044/98‑1 (MQ=255)
                                                                                                          ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     <  2:717871/87‑1 (MQ=255)
                                                                                                          ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     >  1:717871/1‑87 (MQ=255)
                                                                                                          ggCTCTATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCaa                 >  1:21268/1‑139 (MQ=255)
                                                                                                            ctctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagat                                                                                    >  1:159364/1‑70 (MQ=255)
                                                                                                            ctctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                                       >  2:736166/1‑83 (MQ=255)
                                                                                                            ctctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                                                       <  1:736166/83‑1 (MQ=255)
                                                                                                            ctctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     >  2:98344/1‑85 (MQ=255)
                                                                                                            ctctATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     <  1:98344/85‑1 (MQ=255)
                                                                                                            ctctATTTATCCAGAGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagat                                                                                    <  2:159364/70‑1 (MQ=255)
                                                                                                               tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     <  1:780821/82‑1 (MQ=255)
                                                                                                               tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                                                     >  2:780821/1‑82 (MQ=255)
                                                                                                               tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGc                       <  2:221039/128‑1 (MQ=255)
                                                                                                               tATTTATCCAGGGTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGc                       >  1:221039/1‑128 (MQ=255)
                                                                                                                         gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAccc                                                                              >  2:685321/1‑63 (MQ=255)
                                                                                                                         gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAccc                                                                              <  1:685321/63‑1 (MQ=255)
                                                                                                                         gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCaaaa               <  1:734301/126‑1 (MQ=255)
                                                                                                                         gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTc  <  2:456272/139‑1 (MQ=255)
                                                                                                                         gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTc  >  1:866106/1‑139 (MQ=255)
                                                                                                                         gggTCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTc  >  1:195241/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                                       
TTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTC  >  NC_000913/323231‑323490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑