| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,547,986 | C→T | *695* (TAG→TAA) | malQ ← | 4‑alpha‑glucanotransferase (amylomaltase) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,547,986 | 0 | C | T | 96.2% | 74.4 / ‑3.8 | 26 | *695* (TAG→TAA) | malQ | 4‑alpha‑glucanotransferase (amylomaltase) |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (13/12); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01 | |||||||||||
TGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCC > NC_000913/3547859‑3548120 | tgAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTActtcttctt < 1:491746/139‑1 (MQ=255) tGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGc > 2:906492/1‑139 (MQ=255) tGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTgc > 2:197979/1‑139 (MQ=255) acacTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGt < 1:906492/139‑1 (MQ=255) acacTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGt < 1:846843/139‑1 (MQ=255) ctgCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCa > 1:821875/1‑139 (MQ=255) ccGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAAt > 2:919040/1‑139 (MQ=255) aTGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCtt > 2:834126/1‑139 (MQ=255) ccTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCa > 1:286288/1‑139 (MQ=255) ttATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACg < 2:286288/139‑1 (MQ=255) ggCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCcatca < 2:320578/139‑1 (MQ=255) ccTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCatcatc > 2:8475/1‑139 (MQ=255) cGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACAt < 1:129069/139‑1 (MQ=255) tGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACt > 2:644476/1‑139 (MQ=255) ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg > 2:416781/1‑139 (MQ=255) ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg > 1:523129/1‑139 (MQ=255) gTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCg > 2:374703/1‑139 (MQ=255) gTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCg > 2:189204/1‑139 (MQ=255) cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc < 1:639642/139‑1 (MQ=255) atcgcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGttt < 2:821875/139‑1 (MQ=255) gcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTta > 1:513439/1‑139 (MQ=255) catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTtat < 1:197979/139‑1 (MQ=255) atcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTtata < 1:834126/139‑1 (MQ=255) atcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTtata < 1:707161/139‑1 (MQ=255) tCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCAGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAAc < 1:839586/139‑1 (MQ=255) cACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGcc > 2:272414/1‑139 (MQ=255) | TGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCC > NC_000913/3547859‑3548120 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |