Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,567,251	C→T	L261L (TTG→TTA)	glgA ←	glycogen synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,567,251	0	C	T	100.0%	129.7 / NA	41	L261L (TTG→TTA)	glgA	glycogen synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/18);  total (23/18)

TTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCG  >  NC_000913/3567120‑3567385                                                                                                                                    |                                                                                                                                       ttCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTa                                                                                                                                 >  2:823580/1‑139 (MQ=255)  tCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTaa                                                                                                                                >  2:84344/1‑139 (MQ=255)          gTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTc                                                                                                                             >  2:925088/1‑134 (MQ=255)          gTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTc                                                                                                                             <  1:925088/134‑1 (MQ=255)                   cTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTcc                                                                                                               <  1:823580/139‑1 (MQ=255)                                     ggCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGc                                                                                             >  2:164358/1‑139 (MQ=255)                                     ggCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGc                                                                                             >  1:755085/1‑139 (MQ=255)                                                       ttAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGaaa                                                                           <  2:715703/139‑1 (MQ=255)                                                          aGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGAc                                                                        <  2:755085/139‑1 (MQ=255)                                                               cATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCt                                                                   >  1:121422/1‑139 (MQ=255)                                                                aTTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCtt                                                                  >  1:800601/1‑139 (MQ=255)                                                                         tGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGAc                                                         <  2:35026/139‑1 (MQ=255)                                                                         tGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGAc                                                         >  1:35026/1‑139 (MQ=255)                                                                                 gcgcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGt                                                    <  2:309681/136‑1 (MQ=255)                                                                                 gcgcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGt                                                    >  1:309681/1‑136 (MQ=255)                                                                                  cgcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCa                                                <  2:863103/139‑1 (MQ=255)                                                                                  cgcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCa                                                <  1:890663/139‑1 (MQ=255)                                                                                        ttttCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGAc                                          <  1:84344/139‑1 (MQ=255)                                                                                           tCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGAc                                                         >  1:424315/1‑121 (MQ=255)                                                                                           tCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGAc                                                         <  2:424315/121‑1 (MQ=255)                                                                                                 ttATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGtt                                                   <  2:581949/121‑1 (MQ=255)                                                                                                 ttATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATAc                                 <  1:164358/139‑1 (MQ=255)                                                                                                 ttATCTTCCAACGTATCACGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGtt                                                   >  1:581949/1‑121 (MQ=255)                                                                                                              tATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACg                                                        >  2:216427/1‑103 (MQ=255)                                                                                                              tATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACg                                                        <  1:216427/103‑1 (MQ=255)                                                                                                              tATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTg                    >  1:316292/1‑139 (MQ=255)                                                                                                              tATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTg                    >  2:355029/1‑139 (MQ=255)                                                                                                                 cgcgGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGtt                                                                                         <  1:391336/67‑1 (MQ=255)                                                                                                                 cgcgGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGtt                                                                                         >  2:391336/1‑67 (MQ=255)                                                                                                                   cgGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                              <  1:397299/60‑1 (MQ=255)                                                                                                                   cgGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                              >  2:397299/1‑60 (MQ=255)                                                                                                                      gtgtAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCgg            >  2:682322/1‑139 (MQ=255)                                                                                                                            cgcgAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCa                                                <  1:792036/97‑1 (MQ=255)                                                                                                                            cgcgAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCa                                                >  2:792036/1‑97 (MQ=255)                                                                                                                              cgAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                              <  1:445856/49‑1 (MQ=255)                                                                                                                              cgAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                              >  2:445856/1‑49 (MQ=255)                                                                                                                              cgAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcg    >  1:127610/1‑139 (MQ=255)                                                                                                                                aGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGAc                                          <  2:798433/99‑1 (MQ=255)                                                                                                                                aGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGAc                                          >  1:798433/1‑99 (MQ=255)                                                                                                                                aGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcgcg  >  1:918154/1‑139 (MQ=255)                                                                                                                                aGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcgcg  >  1:217277/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                       TTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCG  >  NC_000913/3567120‑3567385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑