Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,587,370	C→T	T143I (ACT→ATT)  *248* (TAG→TAA)	yhhA → ugpQ ←	DUF2756 family protein glycerophosphodiester phosphodiesterase, cytosolic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,587,370	0	C	T	100.0%	56.2 / NA	19	T143I (ACT→ATT)  *248* (TAG→TAA)	yhhA ugpQ	DUF2756 family protein glycerophosphodiester phosphodiesterase, cytosolic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/12);  total (7/12)

GCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGA  >  NC_000913/3587244‑3587470                                                                                                                               |                                                                                                     gcggGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTa                                                                                          <  1:216808/139‑1 (MQ=255)                             ggATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCAt                                                              <  2:685300/139‑1 (MQ=255)                             ggATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCAt                                                              <  1:859497/139‑1 (MQ=255)                                   ttCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTAc                                                        >  2:703890/1‑139 (MQ=255)                                   ttCGTTGAATCAGCAGCATATACTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTAc                                                        <  1:703890/139‑1 (MQ=255)                                          aaTCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCa                                                 <  2:696261/139‑1 (MQ=255)                                           aTCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAattatt                                                                                                  <  1:359433/89‑1 (MQ=255)                                           aTCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAattatt                                                                                                  >  2:359433/1‑89 (MQ=255)                                            tCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGa                                                                                 >  2:166324/1‑105 (MQ=255)                                            tCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGa                                                                                 <  1:166324/105‑1 (MQ=255)                                               gcagcaTATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCca                                            <  1:307638/139‑1 (MQ=255)                                                               gagaggagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGa                                                                                 <  2:199237/86‑1 (MQ=255)                                                               gagaggagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGa                                                                                 >  1:199237/1‑86 (MQ=255)                                                                                             cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa  >  1:223901/1‑135 (MQ=255)                                                                                             cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa  <  2:223901/135‑1 (MQ=255)                                                                                                       cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCa                                                                              <  1:674932/49‑1 (MQ=255)                                                                                                       cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCGGACCa                                                                              >  2:674932/1‑49 (MQ=255)                                                                                                                      cGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATg                                                   <  1:417278/61‑1 (MQ=255)                                                                                                                      cGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATg                                                   <  1:261572/61‑1 (MQ=255)                                                                                                                      cGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATg                                                   >  2:261572/1‑61 (MQ=255)                                                                                                                      cGTTGAAAATTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATg                                                   >  2:417278/1‑61 (MQ=255)                                                                                                                               |                                                                                                     GCGGGATGTTAAACAGCAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGA  >  NC_000913/3587244‑3587470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑