Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	345,649	G→A	*82* (TAG→TAA)	yahM →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	345,649	0	G	A	100.0%	113.3 / NA	36	*82* (TAG→TAA)	yahM	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/18);  total (18/18)

AAAAAGCCCACACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAGAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGCACAGCGGG  >  NC_000913/345513‑345779                                                                                                                                         |                                                                                                                                   aaaaaGCCCACACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTaaag                                                                                                                                  <  1:849404/139‑1 (MQ=255)       cccACACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTaaa                                                                                                                            >  2:321014/1‑139 (MQ=255)                     agTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCg                                                                                                              >  1:579953/1‑139 (MQ=255)                      gTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAGCCTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGc                                                                                                             <  1:670865/139‑1 (MQ=255)                                                 cAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGt                                                                                  <  1:485140/139‑1 (MQ=255)                                                            aTCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAg                                                                                >  2:431749/1‑130 (MQ=255)                                                            aTCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAg                                                                                <  1:431749/130‑1 (MQ=255)                                                            aTCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATac                                                                       >  1:343990/1‑139 (MQ=255)                                                                gAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCa                                                                   <  2:394418/139‑1 (MQ=255)                                                                          ccACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCAc                                                         >  2:135597/1‑139 (MQ=255)                                                                           cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAcc                                                                                                    <  2:143522/95‑1 (MQ=255)                                                                           cACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAcc                                                                                                    >  1:143522/1‑95 (MQ=255)                                                                                     gggggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGa                                              <  1:855235/139‑1 (MQ=255)                                                                                       gggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAccc                                                                                                   <  1:349085/84‑1 (MQ=255)                                                                                       gggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAccc                                                                                                   >  2:349085/1‑84 (MQ=255)                                                                                       gggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACt                                            >  2:781423/1‑139 (MQ=255)                                                                                        ggCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTg                                           >  1:351641/1‑139 (MQ=255)                                                                                          cTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGc                                         >  2:336080/1‑139 (MQ=255)                                                                                           tATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCa                                        <  2:343990/139‑1 (MQ=255)                                                                                            atcatcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCaa                                       >  1:447285/1‑139 (MQ=255)                                                                                                tcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAAcgcg                                   <  1:781423/139‑1 (MQ=255)                                                                                                tcGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAAcgcg                                   >  1:446766/1‑139 (MQ=255)                                                                                                 cGCCGATGGCCGTGATTCTGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTg                                                            >  2:571810/1‑113 (MQ=255)                                                                                                 cGCCGATGGCCGTGATTCTGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTg                                                            <  1:571810/113‑1 (MQ=255)                                                                                                       tgtccttGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATa                            <  2:717504/133‑1 (MQ=255)                                                                                                       tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAc                                                                                                     <  1:753602/66‑1 (MQ=255)                                                                                                       tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCAc                                                                                                     >  2:753602/1‑66 (MQ=255)                                                                                                       tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGa                                              >  1:372701/1‑121 (MQ=255)                                                                                                       tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGa                                              <  2:372701/121‑1 (MQ=255)                                                                                                       tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATa                            <  2:351641/139‑1 (MQ=255)                                                                                                       tGGCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATa                            <  2:446766/139‑1 (MQ=255)                                                                                                         gCCGTGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACg                          <  2:447285/139‑1 (MQ=255)                                                                                                             tGATTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAAGCCGACTGGCAACGCGTTGCa                              >  1:558200/1‑131 (MQ=255)                                                                                                             tGATTCGGCAAATCAGGTTGATTACGTAAAGAGCAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTgga                              <  2:558200/131‑3 (MQ=255)                                                                                                               aTTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGc                                                                                                             >  1:767362/1‑50 (MQ=255)                                                                                                               aTTCGGCAAATCAGGTTGATTACGTAAAGAGTAAATTATCTGCTCACCGc                                                                                                             <  2:767362/50‑1 (MQ=255)                                                                                                                                 aTTACGTAAAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGCACAGCggg  >  2:424248/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                   AAAAAGCCCACACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAGGACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATTACGTAGAGAGTAAATTATCTGCTCACCGCTGCGTCACCCCTTCGTAAATCAGGCGTAGCGCGAATACACCAATAATTGCACCAATAACCCGACTGGCAACGCGTTGCATACGCCCATAAGCACGACGCACAGCGGG  >  NC_000913/345513‑345779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑