Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,352	G→A	R244C (CGC→TGC)	waaH ←	LPS(HepIII)‑glucuronic acid glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,352	0	G	A	100.0%	122.8 / NA	39	R244C (CGC→TGC)	waaH	LPS(HepIII)‑glucuronic acid glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/26);  total (13/26)

GTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTAT  >  NC_000913/3789217‑3789462                                                                                                                                        |                                                                                                               gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                             <  1:862955/139‑1 (MQ=255) gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                             <  1:562485/139‑1 (MQ=255) gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATGTTCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                             <  2:654409/139‑1 (MQ=255)  ttGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTA‑TTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTaa                                                                                                           <  1:437381/139‑1 (MQ=255)            aGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGcaggcaggc                                                                                                             >  2:323061/1‑127 (MQ=255)             gAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAAtata                                                                                                 >  1:140538/1‑139 (MQ=255)               atatCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGt                                                                                               <  2:396014/139‑1 (MQ=255)                   ccGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACg                                                                                           >  2:211945/1‑139 (MQ=255)                   ccGGCTCTTTGCGCACCACATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                             <  2:720247/121‑1 (MQ=255)                   ccGGCTCTTTGCGCACCACATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                             >  1:720247/1‑121 (MQ=255)                      gCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                             <  1:513345/118‑1 (MQ=255)                      gCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                             >  2:513345/1‑118 (MQ=255)                         ctTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATa                                                                                     >  1:34251/1‑139 (MQ=255)                            tGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAAtt                                                                                  >  2:140584/1‑139 (MQ=255)                               gcACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAg                                                                               <  2:175968/139‑1 (MQ=255)                                 aCCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGt                                                                             <  1:211945/139‑1 (MQ=255)                                     cATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                             <  1:829252/103‑1 (MQ=255)                                     cATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                             >  2:829252/1‑103 (MQ=255)                                      aTGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTa                                                                        <  2:692550/139‑1 (MQ=255)                                        ggCAAACACGCAATACCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTAtt                                                                      <  1:553837/139‑1 (MQ=255)                                           aaccacGAAATACCTCGTAAGTTATTTGCTGATGAAATTCAGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTccc                                                                   <  2:926977/136‑1 (MQ=255)                                           aaaCACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGCTTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTccc                                                                   <  2:634953/139‑1 (MQ=255)                                           aaaCACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTccc                                                                   <  2:668582/139‑1 (MQ=255)                                             acacGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                             <  2:791810/95‑1 (MQ=255)                                             acacGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                             >  1:791810/1‑95 (MQ=255)                                             acacGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTAt                                                                       >  2:82109/1‑133 (MQ=255)                                             acacGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTAt                                                                       <  1:82109/133‑1 (MQ=255)                                                        cTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAAc                                                      <  1:882051/139‑1 (MQ=255)                                                               gTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCa                                               <  1:851218/139‑1 (MQ=255)                                                                  aTTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg                                            <  2:140538/139‑1 (MQ=255)                                                                        tgatgaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGtat                                      >  2:770578/1‑139 (MQ=255)                                                                             aaaTTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGc                                 <  2:158149/139‑1 (MQ=255)                                                                                 tCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGat                             <  1:492264/139‑1 (MQ=255)                                                                                 tCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGat                             <  1:219279/139‑1 (MQ=255)                                                                                 tCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCCTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGat                             <  1:745416/139‑1 (MQ=255)                                                                                      aTAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAatat                        >  1:356394/1‑139 (MQ=255)                                                                                                    tGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTg          >  1:851367/1‑139 (MQ=255)                                                                                                            tAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGtat  <  1:770578/139‑1 (MQ=255)                                                                                                                             ctcCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAatat                        >  2:285403/1‑100 (MQ=255)                                                                                                                             ctcCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAatat                        <  1:285403/100‑1 (MQ=255)                                                                                                                                        |                                                                                                               GTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTAT  >  NC_000913/3789217‑3789462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑