Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,819,488 C→T H205H (CAC→CAT
*561* (TAG→TAA
yicG →
ligB ←
UPF0126 family inner membrane protein
DNA ligase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,819,4880CT100.0% 112.9 / NA 36H205H (CAC→CAT
*561* (TAG→TAA
yicG
ligB
UPF0126 family inner membrane protein
DNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

CAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCT  >  NC_000913/3819363‑3819621
                                                                                                                             |                                                                                                                                     
cAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAAc                                                                                                                          <  2:717543/139‑1 (MQ=255)
cAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAAc                                                                                                                          >  1:743684/1‑139 (MQ=255)
                    tgatgTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagc                                                                                                       <  2:220794/138‑1 (MQ=255)
                    tgatgTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagc                                                                                                       >  1:220794/1‑138 (MQ=255)
                      atgTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagccag                                                                                                    >  1:477345/1‑139 (MQ=255)
                          ggTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                          >  2:734971/1‑129 (MQ=255)
                          ggTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                          <  1:734971/129‑1 (MQ=255)
                          ggTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCagccagcc                                                                                                  >  2:278751/1‑137 (MQ=255)
                          ggTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCagccagcc                                                                                                  <  1:278751/137‑1 (MQ=255)
                            tAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACt                                                                                              >  1:547720/1‑139 (MQ=255)
                                                                               gCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgc                                           <  2:154915/139‑1 (MQ=255)
                                                                                  ttAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGTCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGcgcg                                        >  2:546795/1‑139 (MQ=255)
                                                                                  ttAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGcgcg                                        >  1:689818/1‑139 (MQ=255)
                                                                                     aGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTcc                                     <  1:740791/139‑1 (MQ=255)
                                                                                        tGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCa                                                                                                                              >  1:54961/1‑47 (MQ=255)
                                                                                        tGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCa                                                                                                                              <  2:54961/47‑1 (MQ=255)
                                                                                             ttACCGGTCTTTTACAACAGCCACCAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAATTGCCCATCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCTTCCCGATCCAg                             <  2:452110/139‑1 (MQ=255)
                                                                                               aCCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttccttcc                                                              <  1:251369/104‑1 (MQ=255)
                                                                                               aCCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttccttcc                                                              >  2:251369/1‑104 (MQ=255)
                                                                                                ccGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATc                                >  1:806629/1‑133 (MQ=255)
                                                                                                ccGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATc                                <  2:806629/133‑1 (MQ=255)
                                                                                                 cGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTgg                                            >  2:172622/1‑120 (MQ=255)
                                                                                                 cGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTgg                                            <  1:172622/120‑1 (MQ=255)
                                                                                                      ttttACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                         <  1:729120/70‑1 (MQ=255)
                                                                                                      ttttACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                         >  2:729120/1‑70 (MQ=255)
                                                                                                       tttACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAg                   >  1:764923/1‑139 (MQ=255)
                                                                                                         tactacAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAgct                 <  2:689818/139‑1 (MQ=255)
                                                                                                              cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttccttcc                                                              <  2:850188/89‑1 (MQ=255)
                                                                                                              cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttccttcc                                                              >  1:850188/1‑89 (MQ=255)
                                                                                                                gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTgcg                                                                          >  2:142707/1‑75 (MQ=255)
                                                                                                                gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTgcg                                                                          <  1:142707/75‑1 (MQ=255)
                                                                                                                 ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCtgctgc            >  2:210812/1‑136 (MQ=255)
                                                                                                                 ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCtgctgc            <  1:210812/136‑1 (MQ=255)
                                                                                                                   aCGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcc                                          >  1:472757/1‑103 (MQ=255)
                                                                                                                   aCGAAGGACATTAAAGTTCAAAACCTTTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcg                                          <  2:472757/104‑1 (MQ=255)
                                                                                                                        ggACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCt  <  2:764923/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                                     
CAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCT  >  NC_000913/3819363‑3819621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: