Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,853,155	G→A	noncoding (103/140 nt)	istR ←	sRNA antisense regulators IstR‑1 and IstR‑2, affectr tisB, Hfq‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,853,155	0	G	A	100.0%	189.6 / NA	58	noncoding (103/140 nt)	istR	sRNA antisense regulators IstR‑1 and IstR‑2, affectr tisB, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/28);  total (30/28)

AGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTGCGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTT  >  NC_000913/3853029‑3853286                                                                                                                               |                                                                                                                                    aGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAAcc                                                                                                                         >  1:142574/1‑139 (MQ=255)  gCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCg                                                                                                                        >  2:371092/1‑139 (MQ=255)    acaGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCa                                                                                                                      <  1:20200/139‑1 (MQ=255)    acaGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCa                                                                                                                      >  2:571371/1‑139 (MQ=255)         tttACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGca                                                                                                                 <  1:745945/139‑1 (MQ=255)         tttACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGca                                                                                                                 <  2:493919/139‑1 (MQ=255)           tACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                               <  1:371092/139‑1 (MQ=255)           tACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                               <  2:648778/139‑1 (MQ=255)            aCAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCaaa                                                                                                                    >  2:838727/1‑133 (MQ=255)            aCAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCaaa                                                                                                                    <  1:838727/133‑1 (MQ=255)            aCAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCacac                                                                                                              <  2:142574/139‑1 (MQ=255)             cAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACt                                                                                                             <  1:571371/139‑1 (MQ=255)                gggAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCaaa                                                                                                                    >  1:206945/1‑129 (MQ=255)                gggAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCaaa                                                                                                                    <  2:206945/129‑1 (MQ=255)                  gagaCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                               >  2:828740/1‑132 (MQ=255)                  gagaCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                               <  1:828740/132‑1 (MQ=255)                     aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAAcc                                                                                                                         >  2:85936/1‑119 (MQ=255)                     aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAAcc                                                                                                                         <  1:85936/119‑1 (MQ=255)                     aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCaaa                                                                                                                    <  2:637001/124‑1 (MQ=255)                     aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCaaa                                                                                                                    >  1:637001/1‑124 (MQ=255)                     aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCacac                                                                                                              >  1:277480/1‑130 (MQ=255)                     aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCacac                                                                                                              <  2:277480/130‑1 (MQ=255)                     aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATg                                                                                                     >  2:917862/1‑139 (MQ=255)                     aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATg                                                                                                     >  2:870389/1‑139 (MQ=255)                     aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATg                                                                                                     >  1:289269/1‑139 (MQ=255)                     aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATg                                                                                                     >  1:600082/1‑139 (MQ=255)                     aCAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATg                                                                                                     >  1:294555/1‑139 (MQ=255)                            aaaaTTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAacac                                                                                              >  1:670811/1‑139 (MQ=255)                            aaaaTTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAacac                                                                                              >  1:180975/1‑139 (MQ=255)                             aaaTTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACt                                                                                             <  1:917862/139‑1 (MQ=255)                             aaaTTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACt                                                                                             <  2:294555/139‑1 (MQ=255)                                  tttCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAg                                                                                        <  2:15359/139‑1 (MQ=255)                                                     aGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACt                                                                                                             <  1:894277/99‑1 (MQ=255)                                                     aGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACt                                                                                                             >  2:894277/1‑99 (MQ=255)                                                                       gTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCa                                                     >  1:468668/1‑137 (MQ=255)                                                                       gTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCa                                                     <  2:468668/137‑1 (MQ=255)                                                                         aCTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                               >  1:345413/1‑77 (MQ=255)                                                                         aCTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGcaca                                                                                                               <  2:345413/77‑1 (MQ=255)                                                                            gAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAAttt                                              >  2:121754/1‑139 (MQ=255)                                                                             aaTTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAAtttt                                             >  1:844039/1‑139 (MQ=255)                                                                               ttGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCaa                                                                                                 <  2:318915/85‑1 (MQ=255)                                                                               ttGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCaa                                                                                                 >  1:318915/1‑85 (MQ=255)                                                                                tGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGAc                                          >  2:260930/1‑139 (MQ=255)                                                                                  cAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGt                                        >  2:350915/1‑139 (MQ=255)                                                                                  cAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGt                                        >  2:461568/1‑139 (MQ=255)                                                                                     aTAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACgg                                                                                                                                  <  1:16111/46‑1 (MQ=255)                                                                                     aTAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACgg                                                                                                                                  >  2:16111/1‑46 (MQ=255)                                                                                       aaCAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTa                                   >  2:42290/1‑139 (MQ=255)                                                                                       aaCAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTa                                   >  1:354540/1‑139 (MQ=255)                                                                                          aaaaaaCCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTg                                <  1:461568/139‑1 (MQ=255)                                                                                            aaaaCCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCa                              <  1:41485/139‑1 (MQ=255)                                                                                            aaaaCCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCa                              <  2:180975/139‑1 (MQ=255)                                                                                                     ccgGAGCGAGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGt                     <  1:110016/139‑1 (MQ=255)                                                                                                             aGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGt                     <  2:780782/131‑1 (MQ=255)                                                                                                             aGGTTTCGTCAGTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGt                     >  1:780782/1‑131 (MQ=255)                                                                                                                        gTCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGtt  <  2:844039/139‑1 (MQ=255)                                                                                                                         tCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGAc                                          >  2:438463/1‑98 (MQ=255)                                                                                                                         tCGCCTACGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGAc                                          <  1:438463/98‑1 (MQ=255)                                                                                                                               |                                                                                                                                    AGCACAGCTTTACAGGGGAGACAATGGAAAATTTTTCAGCAAGGGAAAATTGAGGGGTTGATCACGTTTTGTACTGAATTGCAGATAACAAAAAACCCCGCCGGAGCGAGGTTTCGTCAGTCGCCTGCGGCTGGTAACCGCAAAGCACACTGTATTATGTCAACACTGAAAGTATACGTGTTCCGCGCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTT  >  NC_000913/3853029‑3853286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑