Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,862,230 G→A pseudogene (1374/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,862,2300GA100.0% 69.8 / NA 23pseudogene (1374/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

CGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTCCCGGCGT  >  NC_000913/3862108‑3862361
                                                                                                                          |                                                                                                                                   
cGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGGGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGACGACCCCACCCAGGGCTTGCAGGa                                                                                                                     <  2:922717/139‑1 (MQ=255)
                                     cGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGc                                                                                >  1:513494/1‑139 (MQ=255)
                                           ccAGGGTTTGTTACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCggt                                                                          >  2:540048/1‑139 (MQ=255)
                                                           gTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAgg                                                                                                                               <  2:894063/70‑1 (MQ=255)
                                                           gTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAgg                                                                                                                               >  1:894063/1‑70 (MQ=255)
                                                            tCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAgg                                                         >  2:616474/1‑139 (MQ=255)
                                                                   gTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGc                                                                                                                             <  1:705841/64‑1 (MQ=255)
                                                                   gTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGc                                                                                                                             >  2:705841/1‑64 (MQ=255)
                                                                     gCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTAtt                                                >  2:673882/1‑139 (MQ=255)
                                                                             tAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCtttt                                        <  2:513494/139‑1 (MQ=255)
                                                                              aaaCGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTg                                       >  1:312789/1‑139 (MQ=255)
                                                                              aaaCGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTg                                       >  2:879440/1‑139 (MQ=255)
                                                                                 cgcgTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGt                                    <  1:616474/139‑1 (MQ=255)
                                                                                   cgTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGCTTGCAGGATACCgg                                                                                                               <  2:926654/62‑1 (MQ=255)
                                                                                   cgTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCACCCAGGGCTTGCAGGATACCgg                                                                                                               >  1:926654/1‑62 (MQ=255)
                                                                                                        ggAGATATTGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCa             >  2:193220/1‑139 (MQ=255)
                                                                                                               ttGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAg                                     >  2:61211/1‑108 (MQ=255)
                                                                                                               ttGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAg                                     <  1:61211/108‑1 (MQ=255)
                                                                                                               ttGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTCCCg      <  1:193220/139‑1 (MQ=255)
                                                                                                                tGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTCCCgg     <  1:879440/139‑1 (MQ=255)
                                                                                                                tGCCGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCAGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTATAGTTTCACTGCCGCATCTTCACGTCCCgg     <  1:620705/139‑1 (MQ=255)
                                                                                                                  ccGACCCCACCCAGGCCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTGTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTCCCGGCg   >  2:620705/1‑139 (MQ=255)
                                                                                                                   cGACCCCACCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTCCCGGCGt  >  1:230116/1‑139 (MQ=255)
                                                                                                                          |                                                                                                                                   
CGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCCCGCCCAGGGCTTGCAGGATACCGGCAGCCTGATCCAGCTCTTTTTTTGGCTCTGCCGCGGTGGTTTGGCCTCGCGAGGCTTTGTATTCGGCTTTTGAGTAGAGTTTCACTTCCGCATCTTCACGTCCCGGCGT  >  NC_000913/3862108‑3862361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: