Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,897,439	G→A	*222* (TAG→TAA)	yieH →	phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,439	0	G	A	100.0%	205.5 / NA	65	*222* (TAG→TAA)	yieH	phosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/32);  total (33/32)

ACGCAGGTATGGAAGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAGTTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTG  >  NC_000913/3897303‑3897569                                                                                                                                         |                                                                                                                                   aCGCAGGTATGGAAGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAAtt                                                                                                                                  >  1:132815/1‑139 (MQ=255)          tGGAAGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACt                                                                                                                         <  2:149800/139‑1 (MQ=255)            gAAGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTcc                                                                                                                       <  2:274944/139‑1 (MQ=255)             aaGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTccc                                                                                                                      <  1:62289/139‑1 (MQ=255)                 gtTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCa                                                                                                                  >  1:855880/1‑139 (MQ=255)                 gtTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCCCGTGGTTGGGATATTACGGAATAATTTTTCAAACTCCCttac                                                                                                                  >  2:67326/1‑137 (MQ=255)                  ttCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcac                                                                                                                            >  1:462825/1‑128 (MQ=255)                  ttCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcac                                                                                                                            <  2:462825/128‑1 (MQ=255)                  ttCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCAc                                                                                                                 >  1:263512/1‑139 (MQ=255)                  ttCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCAc                                                                                                                 >  2:60763/1‑139 (MQ=255)                  ttCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCACTTCAc                                                                                                                 >  2:472134/1‑139 (MQ=255)                            gcgcCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCt                                                                                                       >  1:555351/1‑139 (MQ=255)                                 gACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTa                                                                                                              <  2:73077/127‑1 (MQ=255)                                 gACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTa                                                                                                              >  1:73077/1‑127 (MQ=255)                                              aGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                     <  2:555351/139‑1 (MQ=255)                                                    tCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgtaagta                                                                               >  2:243169/1‑139 (MQ=255)                                                    tCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgtaagta                                                                               >  1:901387/1‑139 (MQ=255)                                                    tCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgtaagta                                                                               >  1:856630/1‑139 (MQ=255)                                                      gTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTaaa                                                                             <  1:243169/139‑1 (MQ=255)                                                      gTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTaaa                                                                             <  2:901387/139‑1 (MQ=255)                                                         cACCCGAAAGTCACCCCCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAggg                                                                          <  1:472134/139‑1 (MQ=255)                                                         cACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAggg                                                                          >  1:915168/1‑139 (MQ=255)                                                         cACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAggg                                                                          >  2:30819/1‑139 (MQ=255)                                                            ccGAAAGTCACCACCTTTACCCATCTTTCGCCATTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGt                                                                       <  1:67326/139‑1 (MQ=255)                                                                aaGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGa                                                                   <  2:915168/139‑1 (MQ=255)                                                                 aGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGat                                                                  <  1:60763/139‑1 (MQ=255)                                                                      ccaccTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCa                                                                                         >  2:200491/1‑111 (MQ=255)                                                                      ccaccTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCa                                                                                         <  1:200491/111‑1 (MQ=255)                                                                      ccaccTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTc                                                             >  2:508988/1‑139 (MQ=255)                                                                               cccATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                    <  2:132815/139‑1 (MQ=255)                                                                                    cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCt                                                                                           >  2:66063/1‑95 (MQ=255)                                                                                    cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCt                                                                                           <  1:66063/95‑1 (MQ=255)                                                                                    cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAgg                                                                           <  2:734874/111‑1 (MQ=255)                                                                                    cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAgg                                                                           >  1:734874/1‑111 (MQ=255)                                                                                       tCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                     <  2:97023/98‑1 (MQ=255)                                                                                       tCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                     >  1:97023/1‑98 (MQ=255)                                                                                              tACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                    >  2:370148/1‑124 (MQ=255)                                                                                              tACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                    <  1:370148/124‑1 (MQ=255)                                                                                               aCCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTg                                                                                                <  2:422309/79‑1 (MQ=255)                                                                                               aCCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTg                                                                                                <  1:294239/79‑1 (MQ=255)                                                                                               aCCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTg                                                                                                >  2:294239/1‑79 (MQ=255)                                                                                               aCCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTg                                                                                                >  1:422309/1‑79 (MQ=255)                                                                                                  tGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGa                                 <  1:619251/139‑1 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTAc                                                                                                             >  1:890805/1‑56 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTAc                                                                                                             <  2:890805/56‑1 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGa                                                                         <  1:488028/92‑1 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGa                                                                         >  2:488028/1‑92 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGata                                                                 <  1:155209/100‑1 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGata                                                                 >  2:155209/1‑100 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGa                                                                   >  1:784065/1‑98 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGa                                                                   <  2:784065/98‑1 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                    >  1:180305/1‑113 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                    <  2:180305/113‑1 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATa                                          >  2:615738/1‑123 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTAtt                          >  2:126035/1‑139 (MQ=255)                                                                                                         tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCGTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATa                                          <  1:615738/123‑1 (MQ=255)                                                                                                          ggAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTc                                                  >  1:820854/1‑114 (MQ=255)                                                                                                          ggAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTc                                                  <  2:820854/114‑1 (MQ=255)                                                                                                             aatcgcgTGGTTTGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGTGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGc                      <  2:132978/136‑1 (MQ=255)                                                                                                             aaGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGc                      <  2:851195/139‑1 (MQ=255)                                                                                                                   gTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGtttt                >  2:197604/1‑139 (MQ=255)                                                                                                                         gggATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGAcc          <  2:856630/139‑1 (MQ=255)                                                                                                                                 aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                     >  2:81019/1‑56 (MQ=255)                                                                                                                                 aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                     <  1:81019/56‑1 (MQ=255)                                                                                                                                 aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTg  >  2:613937/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                   ACGCAGGTATGGAAGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAGTTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTG  >  NC_000913/3897303‑3897569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑