Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,801	A→G	V68A (GTA→GCA)	asnC ←	transcriptional activator of asnA, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,801	0	A	G	100.0%	132.9 / NA	38	V68A (GTA→GCA)	asnC	transcriptional activator of asnA, autorepressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/16);  total (22/16)

GCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGG  >  NC_000913/3926686‑3926927                                                                                                                    |                                                                                                                               gCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCggg                                                                                                         >  2:52827/1‑139 (MQ=255)   tGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCt                                                                                                       >  2:80101/1‑139 (MQ=255)               ttGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcg                                                                                           <  1:52827/139‑1 (MQ=255)               ttGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcg                                                                                           >  1:283127/1‑139 (MQ=255)                         ggCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTa                                                                                 >  2:431986/1‑139 (MQ=255)                            ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATg                                                                              >  1:287438/1‑139 (MQ=255)                                        atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc                                                                                        >  2:647129/1‑117 (MQ=255)                                        atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc                                                                                        <  1:647129/117‑1 (MQ=255)                                             ggCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGc                                                                                                        <  2:35208/96‑1 (MQ=255)                                             ggCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGc                                                                                                        >  1:35208/1‑96 (MQ=255)                                                  tCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCggg                                                                                                         >  2:298253/1‑90 (MQ=255)                                                  tCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCggg                                                                                                         <  1:298253/90‑1 (MQ=255)                                                  tCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTtct                                                        <  1:384091/139‑1 (MQ=255)                                                         ttGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc                                                                                        >  1:303085/1‑100 (MQ=255)                                                         ttGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACgcgc                                                                                        <  2:303085/100‑1 (MQ=255)                                                                  cgGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAAt                                        <  1:80101/139‑1 (MQ=255)                                                                             tCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc                                                                                      >  1:66223/1‑82 (MQ=255)                                                                             tCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGccc                                                                                      <  2:66223/82‑1 (MQ=255)                                                                                  ggCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAgttgct                                                                                                              <  2:541241/53‑1 (MQ=255)                                                                                  ggCGCTCTTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAgctgct                                                                                                              >  1:541241/1‑53 (MQ=255)                                                                                       tctTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAAt                                        >  1:516942/1‑118 (MQ=255)                                                                                       tctTTAATATAATGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTCCTCTACTCGAACGTGAAt                                        <  2:516942/118‑1 (MQ=255)                                                                                                   tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcg                                                                                           <  1:180709/55‑1 (MQ=255)                                                                                                   tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATAcg                                                                                           >  2:180709/1‑55 (MQ=255)                                                                                                   tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGc                                                                  >  2:831964/1‑80 (MQ=255)                                                                                                   tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGc                                                                  <  1:831964/80‑1 (MQ=255)                                                                                                   tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCAtttt                                                          <  2:343476/88‑1 (MQ=255)                                                                                                   tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCAtttt                                                          >  1:343476/1‑88 (MQ=255)                                                                                                   tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg       >  1:580354/1‑139 (MQ=255)                                                                                                   tGCCGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAg       >  1:340205/1‑139 (MQ=255)                                                                                                     ccGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGtt     <  2:144053/139‑1 (MQ=255)                                                                                                     ccGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGtt     >  1:571108/1‑139 (MQ=255)                                                                                                     ccGATAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGtt     >  1:144053/1‑139 (MQ=255)                                                                                                        aTAAAGCAGCCTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTACTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCgg  >  2:470881/1‑139 (MQ=255)                                                                                                                 ccTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCt                                                                 <  1:746028/67‑1 (MQ=255)                                                                                                                 ccTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCt                                                                 >  2:746028/1‑67 (MQ=255)                                                                                                                 ccTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGc          >  2:176114/1‑122 (MQ=255)                                                                                                                 ccTGCGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGc          <  1:176114/122‑1 (MQ=255)                                                                                                                    |                                                                                                                               GCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGG  >  NC_000913/3926686‑3926927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑