Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,937,168 G→A *297* (TAG→TAA rbsB → D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,937,1680GA100.0% 134.3 / NA 42*297* (TAG→TAArbsBD‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/21);  total (21/21)

AACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAA  >  NC_000913/3937035‑3937300
                                                                                                                                     |                                                                                                                                    
aaCTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTa                                                                                                                                 >  2:236270/1‑139 (MQ=255)
aaCTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTa                                                                                                                                 >  2:56142/1‑139 (MQ=255)
  cTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAAt                                                                                                                               <  2:690111/139‑1 (MQ=255)
     gcagcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAg                                                                                                                            <  1:345335/139‑1 (MQ=255)
           aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                                      <  1:906493/139‑1 (MQ=255)
           aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                                      >  1:626469/1‑139 (MQ=255)
             tATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATg                                                                                                                    >  2:794777/1‑139 (MQ=255)
                   tCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGa                                                                                                              >  2:618009/1‑139 (MQ=255)
                       cTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGt                                                                                                          <  1:794777/139‑1 (MQ=255)
                          cccGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGAc                                                                                                       >  2:39981/1‑139 (MQ=255)
                          cccGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGAc                                                                                                       >  2:614460/1‑139 (MQ=255)
                          cccGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGAc                                                                                                       >  1:365939/1‑139 (MQ=255)
                                   aTTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACg                                                                                              <  2:626469/139‑1 (MQ=255)
                                    ttGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGt                                                                                             >  2:383871/1‑139 (MQ=255)
                                       gcgcGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAt                                                                                          <  1:614460/139‑1 (MQ=255)
                                         gcgAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCg                                                                                        <  2:525154/139‑1 (MQ=255)
                                             aaGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAg                                                                                    >  2:886636/1‑139 (MQ=255)
                                                 cGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                                <  2:660417/139‑1 (MQ=255)
                                                 cGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                                <  2:661245/139‑1 (MQ=255)
                                                      aaaCCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                           <  2:617069/139‑1 (MQ=255)
                                                      aaaCCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                           <  2:365939/139‑1 (MQ=255)
                                                        aCCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTa                                                                         <  2:922196/139‑1 (MQ=255)
                                                                 aaaGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTaa                                                                                                                                >  1:879890/1‑75 (MQ=255)
                                                                 aaaGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTaa                                                                                                                                <  2:879890/75‑1 (MQ=255)
                                                                        tGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAg                                                         >  1:154597/1‑139 (MQ=255)
                                                                               cGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCAcgc                                                  >  2:822022/1‑139 (MQ=255)
                                                                                 agaAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                                <  1:618009/139‑1 (MQ=255)
                                                                                 agaAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                                <  1:39981/139‑1 (MQ=255)
                                                                                    aaGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCAc                                             <  1:886636/139‑1 (MQ=255)
                                                                                    aaGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCAc                                             <  1:236270/139‑1 (MQ=255)
                                                                                                      ctgTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGAc                                                                                                       <  1:418619/61‑1 (MQ=255)
                                                                                                      cGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGAc                                                                                                       >  2:418619/1‑63 (MQ=255)
                                                                                                           gATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgt                                                                            <  2:588583/85‑1 (MQ=255)
                                                                                                           gATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgt                                                                            >  1:588583/1‑85 (MQ=255)
                                                                                                            aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGc                                                           <  1:84242/101‑1 (MQ=255)
                                                                                                            aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGc                                                           >  2:84242/1‑101 (MQ=255)
                                                                                                                  aaCTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAaca                                      <  2:467898/116‑1 (MQ=255)
                                                                                                                  aaCTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAaca                                      >  1:467898/1‑116 (MQ=255)
                                                                                                                       gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGaa          >  2:805963/1‑139 (MQ=255)
                                                                                                                       gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGaa          >  1:530116/1‑139 (MQ=255)
                                                                                                                           ttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATg      >  2:499150/1‑139 (MQ=255)
                                                                                                                               gCAGTAATTTTAATCAGGTTGTATGACCTGAGGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCaaa  >  1:77035/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                    
AACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAA  >  NC_000913/3937035‑3937300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: